The bubbling of H2O2 frozen tissue is caused by the chemical destruction of the tissues. Do you remember when you apply 3% H2O2 to a skin wound? Don't you remember the bubbling it produces? That is the oxygen destruction of the white blood cells that constitute the pus in any wound, as well as the chemical destruction (oxidation) of the live skin cells surrounding the wound. The addition of methanol does not affect the oxidation of the peroxidase. It is just "a way of doing things" those things that we use to do without any specific (scientific) reason. René J.
________________________________ From: "Lewis, Patrick" <patrick.le...@seattlechildrens.org> To: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu> Sent: Friday, July 13, 2012 12:16 PM Subject: [Histonet] H2O2 Recep So what I'm gathering from all this. Thanks by the way for the discussion. Methanol is unnecessary for the H2O2, reaction, but it can make it slightly faster since both methods will be working in concert. If you have tissue that has more peroxidase than usual, then extra time is required. Also, If you use straight aq H2O2 on frozen sections the bubbling may damage the tissue. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
