I have some old H&E slides that I would like to try IHC staining on. I am having trouble keeping the tissue on the slide. I put a slide in xylene to remove the cover slip and eventually the section floated off the slide when I tried to rehydrate. The second time I tried Mount-Quick from Newcomer Supply and was able to put the section on a charged slide. The section stayed on the slide but was loose after hydration. It was just hanging on after retrieval and did not stain well. I have a limited number of slides. If anyone has experience in this area and knows the best way to keep tissue on a slide I would really appreciate your advice. Thanks in advance.
Kathryn Stoll, HT(ASCP) Department of Pathology Medical College of Wisconsin 9200 W Wisconsin Ave Milwaukee WI 53226 414.805.1525 kst...@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
