For this difficult tissue, and for any other for that matter, add to your regular round water bath at 45ºC, 2 mL of liquid dish washer soap. The soap will reduce the surface tension of the water and will allow the sections to extend completely, eliminating any folds. René J.
________________________________ From: Jennifer Sipes <jengirl1...@yahoo.com> To: Histonet <histonet@lists.utsouthwestern.edu> Sent: Friday, August 24, 2012 8:50 AM Subject: [Histonet] folds in tissue Good morning/afternoon/evening Histonetters! I'm having a problem with my tissue folding. It's tiny folds in mouse tissue that are too little to see without the use of a microscope. I was wondering if anyone has any tricks that might eliminate this so I can ensure that I'm turning out the best quality slides without having to review every slide I turn out under the microscope. I'm using distilled water in my water bath and it's set to about 40 degrees Celcius. All help is appreciated! Thank you all so much for your time! Regards, Jen Jennifer K. Sipes, ALAT Sr. Laboratory Technician Johns Hopkins University Ross 933 720 Rutland Avenue Baltimore, MD 21205 phone: 410-614-0131 fax: 410-955-9677 cell: 443-631-6361 e-mail: jsip...@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
