Tora If you are using an H&E it was vertually impossible in our hands to try to threshold fiber area. We worked with Image Pro Plus for about 6 months trying to get this to work and we could not, we ended up creating an algorithm in image pro that allowed us to trace the fibers and that would calculate fiber area and diameter, etc.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tora Bardal [tora.bar...@bio.ntnu.no] Sent: Thursday, September 06, 2012 5:48 AM To: mail=histonet@lists.utsouthwestern.edu Subject: [Histonet] ImageJ : start help for muscle fiber count Any guru out there that might give me some ideas how to measure size and number of muscle fibers in a cross section using ImageJ Area by using freehand tool works................. but I'm having problems making a threshold with the muscle cells outlined for automatic measurements. I'm not experienced, might be I haven't found the correct tool :-) Picture sample: http://www.nt.ntnu.no/users/tbardal/muscle/muscle%20paraffin%202%c2%b5%2016x.jpg _________________________________________________________________________________ Tora Bardal Overingeniør/ Senior Engineer NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
