Hazel, The only time I have seen any problem was when a stain was not fully washed off the slide. Once you put the slide in xylene and coverslip it the slide is just like any other.
Amos On Sep 12, 2012 8:57 AM, "Horn, Hazel V" <hor...@archildrens.org> wrote: > I suppose my age will show here but I was always taught to NEVER let > slides dry out unless the procedure indicated such. Is there no drying > artifact when you let these slide dry before coverslipping? > > Hazel Horn > Supervisor of Histology/Autopsy/Transcription > Anatomic Pathology > Arkansas Children's Hospital > 1 Children's Way | Slot 820| Little Rock, AR 72202 > 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax > hor...@archildrens.org > archildrens.org > > > > > 100 YEARS YOUNG! > JOIN THE PARTY AT > ach100.org > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks > Sent: Tuesday, September 11, 2012 7:31 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] air drying special stain slides rather than dehydrate > and clear > > Hi, > My choice to air dry rather than dehydrate in ETOH & xylene is based > on the stain rather than the spooky xylene hazard boogyman. Yes, not using > xylene if it is not really needed is not a bad idea, but the main reason I > air dry some stains is the alcohols remove some of the stains. Ever have a > beautiful Luxol Fast Blue bleach out on you? The most exasperating thing in > the world! > Generally stains that end in water can easily be air dried. Something > alcoholic like eosin or Movat's Pentachrome ending in alcoholic saffron > might as well be finished traditionally. I air dry any stain that is > counterstained in Nuclear Fast Red, Light Green, Methyl Green. I have air > dried IHCs with no ill effects too. Don't try it with fluorescents though, > that would be bad ... and pointless. > I don't put them in an oven. I set them at the front of the fume hood > and go do something else for a few minutes. If I want to rush it I close > the sash to increase the flow rate for a bit. (Of course it is opened back > up right after so the draft works properly.) Amos > > > On Tue, Sep 11, 2012 at 11:09 AM, < > histonet-requ...@lists.utsouthwestern.edu > > wrote: > > > Message: 16 > > Date: Tue, 11 Sep 2012 10:32:08 -0400 > > From: "Diana McCaig" <dmcc...@ckha.on.ca> > > Subject: [Histonet] air drying special stain slides rather than > > dehydrate and clear > > To: <histonet@lists.utsouthwestern.edu> > > Message-ID: > > <dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca> > > Content-Type: text/plain; charset="us-ascii" > > > > I was hoping to get information on why special stains are dehydrated, > > cleared and mounted vs allowing them to be blotted dry, air dried then > > coverslip. > > > > > > > > Every procedure I have ever encountered always indicates to dehydrate > > and clear but I have heard where some labs are blotting the slides , > > allowing to air dry (probably not set standard time) and dipped in > > xylene prior to cover slipping. Reason given is that the counterstain > > gets washed out. Wouldn't adjusting the times be a better resolution. > > > > > > > > I understand residual water could be present and cause long term > > issues on storage but wanted some other opinions on this process. > > > > > > > > Diana > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ****************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************** > The information contained in this message may be privileged and > confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to the message and deleting it from your > computer. > Thank you. > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet