By far the best ab for endothelial cells in mouse tissue is rat anti CD31 from Dianovo, it picks up the really early forming vessels better than any other cd31 or F8 or SMA I have ever used. We like it in AP/red.
Regards, Patsy Hi, My favorite by far is VonWillebrandt (spelling?) factor VIII. Dako has a really good one (Cat# A0082) that works great in mouse tissue. CD31 is OK but it is really finicky. CD34 works but F8 is easier and more reliable. Amos On Wed, Sep 5, 2012 at 1:01 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 5 > Date: Wed, 5 Sep 2012 10:04:58 +0000 > From: "Bruijntjes, J.P. (Joost)" <joost.bruijnt...@tno.triskelion.nl> > Subject: [Histonet] endothelial cell marker > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <a3a9d8b3f110d44fa1022b49913668668835b...@exc-mbx03.tsn.tno.nl> > Content-Type: text/plain; charset="us-ascii" > > Hi all > > Is anyone of you familiar with an antibody directed against endothelial > cells which can be applied on FFPE mouse tissues? > > Best regards > Joost Bruijntjes > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet