Fix for a week or two, then rinse out most of the picrates with 50-70%
ethanol. Fixation preserves cell morphology, it does not distort it.
Geoff
On 9/14/2012 9:06 AM, Margaret Horne wrote:
Hello Everyone, I am asking this for a friend.
How long can mouse testis be kept in Bouins without distortion of cell
morphology? Days? weeks? months? years?
I noticed in the Archives that many people fix in Bouins , rinse, then
store in 70% EtOH. This is preferable I assume. Again, how long is ok?
Thanks in advance for the sharing of your accumulated wisdom,
Margaret
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcaul...@umdnj.edu
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