&nb= sp;I have used Pharmingen anti cd31but make no mistake this can be finicky = and it would appear it should not be.
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC On 09/06/12, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to [1]= histonet@lists.utsouthwestern.edu To subscribe or unsubscribe vi= a the World Wide Web, visit [2]http://lists.u= tsouthwestern.edu/mailman/listinfo/histonet or, via email, send a me= ssage with subject or body 'help' to [3]histonet-requ= e...@lists.utsouthwestern.edu You can reach the person managing t= he list at [4]histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specif= ic than "Re: Contents of Histonet digest..." Today's Topics:<= BR> 1. Re: Histonet Digest, Vol 106, Issue 4 (Madeleine Huey) 2. Ergo= nomics (Gagnon, Eric) 3. endothelial cell marker (Amos Brooks) 4. der= matologist volunteer needed - Belize Mission Project (Grantham, Andrea L= - (algranth)) 5. ImageJ : start help for muscle fiber count (Tora Barda= l) 6. RE: ImageJ : start help for muscle fiber count (Elizabeth Chlip= ala) 7. Canton, Ohio HT Position (Hale, Meredith) 8. Colorado HT (Hal= e, Meredith) --------------------------------------------------- ------------------- Message: 1 Date: Wed, 5 Sep 2012 11:37:49 -07= 00 From: Madeleine Huey <[5]madeleineh...@gmail.com> Subje= ct: [Histonet] Re: Histonet Digest, Vol 106, Issue 4 To: [6]h= isto...@lists.utsouthwestern.edu Message-ID: <CAF2e4CK7SUq0R+5 +6koeqPsF+792q6[7]7x5pbw4s0strp8gy-...@mail.gmail.com>< BR>Content-Type: text/plain; charset=ISO-8859-1 > Date: Wed, 5 = Sep 2012 10:04:58 +0000 > From: "Bruijntjes, J.P. (Joost)" <[8]joost.bruijnt...@tno.triskelion.nl> > Subject: [Hist= onet] endothelial cell marker > To: "[9]histo...@lists.uts= outhwestern.edu" > <[10]Histonet@lists.utsouthwester= n.edu> > Message-ID: > <A3A9D8B3F110D44FA102[11]2b49913668668835b...@exc-mbx03.tsn.tno.nl> > Con= tent-Type: text/plain; charset="us-ascii" > > Hi all ><= BR>> Is anyone of you familiar with an antibody directed against endothe= lial cells which can be applied on FFPE mouse tissues? > > Best= regards > Joost Bruijntjes Joost, AbCam sell Rabbit anti-CD31 = & will cross-react with Mouse FFPE tissues (Cat. # ab28364); [12]http://www.abcam.com/CD31-antibody-ab28364.html Hope= this help! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - El Camino= Hospital (Pathology) ------------------------------ = Message: 2 Date: Wed, 5 Sep 2012 19:56:21 +0000 From: "Gagnon, Eric" = <[13]gagn...@kgh.kari.net> Subject: [Histonet] Ergonomics To: = "[14]histonet@lists.utsouthwestern.edu" <[15]= histonet@lists.utsouthwestern.edu> Message-ID: <5F06C3AD0B2 7264CA20C[16]fa986c87882e31519...@exchangepv3.kgh.on.ca<= /A>> Content-Type: text/plain; charset="us-ascii" Hi Karen,<= BR> Here's a response to your question, as I haven't seen any others (?) While I haven't used the Newcomer handgrip device, I developed have DeQuer= vain's Tenosynovitis , a repetitive strain injury from turnin' the ol' micr= otome wheel for 28 or so years now. I would definitely recommend the foot p= edal, since you have a microtome equipped with one. Yes, there is some loss= of 'feeling' and it's a change from two hands on, but there is still the o= ption of taking the microtome 'off line' and turning the wheel manually for= difficult blocks. I can only speak for myself, but the more I used = the foot pedal, the more confident and at ease I became. Start off slowly, = working up towards more challenging blocks as soon as you can. Not that one= can ever let one's guard down with anything with a motor...car, microtome,= what have you. But in terms of ergonomics and saving your joints, I= would try anything and everything you have at your disposal. It's never to= o late to change for the better. Hope this helps, Eric Gagnon= MLT Histology Laboratory Kingston General Hospital, Kingston, Ont= ario, Canada ------------------------------ Message: 3 Date: Wed, 5 Sep 2012 17:06:12 -0400 From: Amos Brooks <[17]amosbrooks@= gmail.com> Subject: [Histonet] endothelial cell marker To: [18]histonet@lists.utsouthwestern.edu, [19]joost.b= ruijnt...@tno.triskelion.nl Message-ID: <CAC95ki8RbfYwzBCyF_H4 gaNgRWDJ+x[20]mgmp1oc7=wqz8_s3m...@mail.gmail.com>Content-Type: text/plain; charset=ISO-8859-1 Hi, My favorite = by far is VonWillebrandt (spelling?) factor VIII. Dako has a really good= one (Cat# A0082) that works great in mouse tissue. CD31 is OK but it is= really finicky. CD34 works but F8 is easier and more reliable. Amos On Wed, Sep 5, 2012 at 1:01 PM, <hi= stonet-requ...@lists.utsouthwestern.edu>wrote: > Message: = 5 > Date: Wed, 5 Sep 2012 10:04:58 +0000 > From: "Bruijntjes, J= .P. (Joost)" <[21]joost.bruijnt...@tno.triskelion.nl><= BR>> Subject: [Histonet] endothelial cell marker > To: "[22]Histonet@lists.utsouthwestern.edu" > <[23]Hist= o...@lists.utsouthwestern.edu> > Message-ID: > <A3A9D= 8B3F110D44FA102[24]2b49913668668835b...@exc-mbx03.tsn.tno .nl> > Content-Type: text/plain; charset="us-ascii" ><= BR>> Hi all > > Is anyone of you familiar with an antibody d= irected against endothelial > cells which can be applied on FFPE mous= e tissues? > > Best regards > Joost Bruijntjes > ------------------------------ Message: 4 Date: Wed, 5 S= ep 2012 23:11:53 +0000 From: "Grantham, Andrea L - (algranth)" <[25]algra...@email.arizona.edu> Subject: [Histonet] dermatologist v= olunteer needed - Belize Mission Project To: HISTONET <[26]histonet@lists.utsouthwestern.edu> Message-ID: <E95B5342= 1770534BA4[27]0FE080641B847E1FF105@Carousel.catnet .arizona.edu> Content-Type: text/plain; charset="us-ascii" <= BR>If anybody works for a dermatologist or knows of one or IS one who might= like to volunteer for ONE WEEK in Belize in November please let me know.A family emergency has required our dermatologist to cancel his trip this= year. The Belize MIssion Project is a dental/medical mission that is in= its 20th year and is set up to provide dental and health care to the peopl= e of Belize who can't afford these services - in the cities, the remote May= an villages and on the islands. It is a rewarding week among wonderful p= eople who come from all over the US and Canada to volunteer. Dermatology is= very similar to what we see here in the US (melasma, psoriasis, alopecia, = & vitiligo with more scabies, fungus and a leshmaniasis or two thrown i= n for good measure). The Belizeans are friendly and appreciative, the co= untry is beautiful, food is delicious and there is some time for activities like fishing and snorkeling too! English is the official language and one = US dollar is equal to two belizeans. We headquarter on Ambergris Caye an= d travel via Tropic Air to various parts of the country. Keeping my fing= ers crossed that a willing dermatologist is out there. Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialis= t University of Arizona Cellular and Molecular Medicine Histology = Service Laboratory P.O.Box 245044 Tucson, AZ 85724 [28]algr a...@email.arizona.edu<[29]mailto:algra...@email.arizona.edu&g= t; Tel: 520.626.4415 Fax: 520.626.2097 ------------------= ------------ Message: 5 Date: Thu, 6 Sep 2012 11:48:10 +0000 F= rom: Tora Bardal <[30]tora.bar...@bio.ntnu.no> Subject: [Histo= net] ImageJ : start help for muscle fiber count To: "[31]mail=histonet@lists.utsouthwestern.edu" <[32]histon= e...@lists.utsouthwestern.edu> Message-ID: <FBB97A1AA0A8494CA 8E1[33]1f7b951f3a2e13a6f...@warehouse03.win.ntnu.no> Content-Type: text/plain; charset="iso-8859-1" Any guru o= ut there that might give me some ideas how to measure size and number of mu= scle fibers in a cross section using ImageJ Area by using freehand tool = works................. but I'm having problems making a threshold with the = muscle cells outlined for automatic measurements. I'm not experienced, m= ight be I haven't found the correct tool :-) Picture sample: [34]http://www.nt.ntnu.no/users/tbard al/muscle/muscle%20paraffin%202%c2%b5%2016x.jpg ________________ _________________________________________________________________ Tora B= ardal Overingeni�r/ Senior Engineer NTNU Senter for fiskeri o= g havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim ------------------------------ Message: 6 Da= te: Thu, 6 Sep 2012 07:10:02 -0600 From: Elizabeth Chlipala <[35]liz@premi= erlab.com> Subject: [Histonet] RE: ImageJ : start help for muscle= fiber count To: Tora Bardal <[36]tora.bar...@bio.ntnu.no>, "[37]mail=histonet@lists.utsouthwestern.edu" <= ;[38]histonet@lists.utsouthwestern.edu> Message-ID: = <14E2C6176416974295479C64[39]A11CB9AE0162D07D5C44@S BS2K8.premierlab.local> Content-Type: text/plain; charset="iso-= 8859-1" Tora If you are using an H&E it was vertually imp= ossible in our hands to try to threshold fiber area. We worked with Image P= ro Plus for about 6 months trying to get this to work and we could not, we = ended up creating an algorithm in image pro that allowed us to trace the fi bers and that would calculate fiber area and diameter, etc. Liz <= BR>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC P= O Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763= cell (303) 682-9060 fax [40]l...@premierlab.com Ship to address= : Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, = CO 80504 ________________________________________ From: [41]histonet-boun...@lists.utsouthwestern.edu [[42]histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tora Bardal= [[43]tora.bar...@bio.ntnu.no] Sent: Thursday, September 06, 2012 5:= 48 AM To: [44]mail=histonet@lists.utsouthwestern.edu<= /A> Subject: [Histonet] ImageJ : start help for muscle fiber count Any guru out there that might give me some ideas how to measure size and = number of muscle fibers in a cross section using ImageJ Area by using fr= eehand tool works................. but I'm having problems making a thresho= ld with the muscle cells outlined for automatic measurements. I'm not ex= perienced, might be I haven't found the correct tool :-) Picture sample: [45]http://www.nt.ntnu.no /users/tbardal/muscle/muscle%20paraffin%202%c2%b5%2016x.jpg ____ ______________________________________________________________________ _____= __ Tora Bardal Overingeni�r/ Senior Engineer NTNU Senter f= or fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 = Trondheim _______________________________________________ Histonet mailing list [46]Histonet@lists.utsouthwestern.edu [47]http://lists.utsouthwestern.edu/mailman= /listinfo/histonet ------------------------------ Message: 7 Date: Thu, 6 Sep 2012 14:29:03 +0000 From: "Hale, Meredit= h" <[48]mh...@miracals.com> Subject: [Histonet] Canton, Ohio HT Pos= ition To: "[49]Histonet@lists.utsouthwestern.edu" <<= A class=parsedEmail href="mailto:Histonet@lists.utsouthwestern.edu"; tar get=_blank>Histonet@lists.utsouthwestern.edu> Message-ID: &l= t;0E828EC51C7CC445A5[50]1E53F81B64E8C7 02BE09@s-irv-exchmb.PathologyPartners.intranet> Content-Type:= text/plain; charset="us-ascii" Great part time opportunities' for= Histotechnician's in Canton , Ohio! Gastroenterology Associates is looking= for certified HT's or HTL's to join their new laboratory . Candidate must = meet the following criteria: * Meet CLIA Grossing Requirements : CFR= 493.1489, [51]http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior e= xperience grossing GI specimens * HT ASCP Certified Duties includ= e: * Grossing * Embedding * Microtomy * Stainin= g * Ability to be flexible and take on additional duties' as needed< BR> This is a part time that offers a competitive rate and flexible hours. Interested applicants should contact Meredith Hale; phone 214-596-22= 19 or through email [52]mh...@miracals.com<[53]mailto:mh...@miracals.com>= ; Meredith Hale HT (ASCP)cm Operations Liaision Director and = Education Coordinator Miraca Life Sciences 6655 North MacArthur B= lvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253<= BR>Fax: 1-866-688-3280 [54]mh...@miracals.com = ------------------------------ Message: 8 Date: Thu, 6 Sep 2012 1= 4:29:40 +0000 From: "Hale, Meredith" <[55]mh...@miracals.com> Su= bject: [Histonet] Colorado HT To: "[56]Histonet@lists.utsouth= western.edu" <[57]Histonet@lists.utsouthwestern.edu= > Message-ID: <0E828EC51C7CC445A5[58]1E53F81B64E8C702BE23@s-irv-exchmb.PathologyPart ners.intranet= > Content-Type: text/plain; charset="us-ascii" Great par= t time opportunities' for Histotechnician's in Centennial, Colorado! Colora= do GI Pathology is looking for certified HT's or HTL's to join their existi= ng laboratory staff. Candidate must meet the following criteria: * M= eet CLIA Grossing Requirements : CFR 493.1489, [59]http://wwwn.cd= c.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * HT ASCP Certified Duties include: * Grossing * Embeddi= ng * Microtomy * Staining * Ability to be flexible and= take on additional duties' as needed This is a part time that o= ffers a competitive rate and flexible hours. Interested applicants should c= ontact Meredith Hale; phone 214-596-2219 or through email [60]mh...@miracals.com <[61]mailto:mh...@miracals.com> Meredith Hale HT (ASCP)c= m Operations Liaision Director and Education Coordinator Miraca L= ife Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Offic= e: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 [62]mhale@miraca= ls.com ------------------------------ _______________________________________________ Histonet mailing list[63]Histonet@lists.utsouthwestern.edu [64]http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 106, Issue 5 *************************= *************** References 1. 3D"mailto:histonet@lists.utsouthwestern.edu"; 2. 3D"http://lists.u=/ 3. file://localhost/tmp/3D"mail 4. 3D"mailto:histonet-ow...@lists.ut 5. 3D"mailto:madele 6. 3D"mailto:histonet@lists.utsouthwestern.edu"; 7. 3D"mailto:7X5PBw4S0STRp8GY-wRg@m 8. 3D"mailto:joost.bruijnt...@tno.triskelion.nl"; 9. file://localhost/tmp/3D"m 10. 3D"mailto:Hist 11. 3D"mailto:2b49913668668835b...@exc-mbx03.tsn.tno.nl"; 12. 3D"http://www.abcam.com/CD31-antibody-ab28364.html"; 13. 3D"mailto:gagn...@kgh.kari.net"; 14. 3D"mailto:histonet@lists.utsouthwestern.edu"; 15. 3D"mailto:histonet@lists.utsouthwestern.edu"; 16. 3D"mailto:FA986C87882E3151998F@EXCHANG 17. 3D"mailto:amosbro...@gmail.com"; 18. 3D"mailto:histonet@lists.utsouthwestern.edu"; 19. 3D"mailto:joost.bruijnt...@tno.triskelion.nl"; 20. 3D"mailto:mgmp1oc7=WqZ8_S3mCdQ@mail 21. 3D"mailto:joost.bruijntjes@tno 22. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 23. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 24. 3D"mailto:2B49913668668835BE43@E 25. 3D"mailto:algra...@email.arizona.edu"; 26. 3D"mailto:histonet@lists.utsouthwestern.edu"; 27. 3D"mailto:0FE080641B847E1FF105@Carous 28. 3D"mailto:algra...@email.arizona.edu"; 29. 3D"mailto:algranth 30. 3D"mailto:tora.bardal@bio. 31. 3D"mailto:mail=histonet@lists.utsouthwestern.edu"; 32. 3D"mailto:histonet@lists.utsouthwestern.edu"; 33. 3D"mailto:1F7B951F3A2E13A6F452@WAREHOUSE03.w 34. 3D"http://www.nt.ntnu.no/users/tbardal/muscle/muscle%20para 35. 3D"mailto:l...@premierlab.com"; 36. 3D"mailto:t 37. 3D"mailto:mail=histonet@lists.utsouthwester 38. 3D"mailto:histonet@lists.utsouthwestern.edu"; 39. 3D"mailto:A11CB9AE0 40. 3D"mailto:liz@pr 41. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu"; 42. 3D"mailto:histonet-boun...@lists.utsouthwestern.edu"; 43. 3D"mailto:tora.bar...@bio.ntnu.no"; 44. 3D"mailto:mail=histo...@lists.ut 45. 3D"http://www.nt.ntnu.no/users/tbardal/muscle/m 46. 3D"mailto:Histonet@li 47. 3D"http://lists.utsouthwestern.edu/mailman 48. 3D"mailto:mh...@miracals.com"; 49. 3D"mailto:Histonet@lists.utsouthw 50. 3D"mailto:1E53F81B64E8C702B 51. 3D"http://wwwn.cdc.gov/clia/regs/toc. 52. 3D"mailto:mh...@miracals.co 53. file://localhost/tmp/3D" 54. 3D"mailto:mhale@mirac 55. file://localhost/tmp/3D" 56. file://localhost/tmp/3D"mailt 57. 3D"mailto:Histonet@list 58. file://localhost/tmp/3D 59. ="http://wwwn.cdc.gov/clia/regs/toc.aspx/"; 60. 3D"mailto:mh...@miracals.com"; 61. 3D"mailto:mh...@miracals.com"; 62. 3D"mailto:mh...@miracals.com"; 63. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 64. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
