Diana-
We cut routine slides at 4 microns.
Blocks marked for lymph node are cut at 2.5.
Prostate needle cores: we cut three levels and save two unstained at each level.
H&E has two sections - bottom section is always deepest - unstains are
taken from in between the upper and lower section on the H&E.
Nancy
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Message: 3
Date: Tue, 18 Sep 2012 14:00:22 -0400
From: "Diana McCaig" <dmcc...@ckha.on.ca>
Subject: [Histonet] thickness of slides
To: <histonet@lists.utsouthwestern.edu>
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<dcfd9e6a390e294aaf3a2561cd32e5c417a90...@ckhamail1.ckha.on.ca>
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Can you tell me what thickness you cut your routine slides for H&E and immuno
(in particular lymph nodes).
Also, your protocol for cutting prostate needle core biopsies.....how many
spares you cut and do you designate the level on the slide label if multiple
levels are submitted on one slide?
Thanks
Diana
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