Our back up died, and administration, in a thought of saving money, decided to not get it repaired as we only get about 5 frozens/week. Well, I got called in on a Saturday for a frozen, and, yup, you guessed it.......the brand new one was room temperature!! (housekeeping mistakenly turned it off) I turned it on, ran and put the blade holder in the -70 freezer in blood bank, the blades in our histobath (isopentane freezer) and prayed for an hour until the frozen came down. I could only get about 2 swipes on the tissue before it started sliding off the chuck! Removed it and re-froze it until I was able to get a section!! What a day. Administration decided to repair the back up!! I vote for a back up!
Lynette ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of barbara.cr...@lpnt.net [barbara.cr...@lpnt.net] Sent: Friday, September 21, 2012 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] back up cryostat I would like to know what the consensus is on having a backup cryostat. Does anyone have a back up cryostat? I am nervous about not having a backup cryostat Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY X5451 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
