Dear Histonetters, Has anyone out there experienced problem with optimizing PanCK MNF116 antibody? Our CKMNF116 stains dendritic cells on lymph nodes using Ventana protocol: CC1 - 8 mins, Ab incubation time 16 mins, Post primary peroxidase inhib selected. Basically, it should stain cytokeratins, but it stains dendritic cells as well. What would be the possible causes and solutions. Any suggestions would be greatly appreciated. Many thanks.
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