Hello all, Anyone arriving in Vancouver airport around 4 to 5pm that would like to share a limo over to the convention center arear? If 3 or 4 of us can combine, its actually cheaper than a cab? And I'm sure a much more enjoyable ride! Jeanne Clark (SFO to Vancouver)
From: "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 31, 1969 4:00 PM Subject: Histonet Digest, Vol 106, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Have Blocks Will Cut (Contact HistoCare) 2. ShurMount coverslipper (Charles, Roger) 3. Question (Richard Cartun) 4. Ferret lungs (Blair, Paula) 5. Come and visit the Legislative Committee in Vancouver! (Colleen Forster) 6. RE: softening agent for tissues embedded in paraffin blocks (Debbie Faichney) 7. Re: Ferret lungs (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 26 Sep 2012 12:17:17 -0500 From: Contact HistoCare <cont...@histocare.com> Subject: [Histonet] Have Blocks Will Cut To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <2eebc9db-153f-4bb0-9f92-9587f3ac3...@histocare.com> Content-Type: text/plain; charset=us-ascii Thanks to everyone who inquired about contracting my services and gave me an opportunity to explain the value I add. This is a great opportunity for those involved and it keeps the stress to a minimum and puts people in a better mood since the individual workload is lighter and you don't feel like a machine. For the next few months, my reach will remain in the southern states unless I'm compelled to do otherwise :). Please contact me if you need some relief in your lab, I know many do but may not want to admit it :) Thanks again histonet ------------------------------ Message: 2 Date: Wed, 26 Sep 2012 13:58:25 -0400 From: "Charles, Roger" <rchar...@pa.gov> Subject: [Histonet] ShurMount coverslipper To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3809c163dc1da54aa534b3c7794d07b6ca7382d...@enhbgmbx01.pa.lcl> Content-Type: text/plain; charset="us-ascii" Hello, Does anyone have experience with the ShurMark glass coverslipper. We are looking at purchasing one and I would like to hear pros and cons. Thanks Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us<http://www.agriculture.state.pa.us/> ------------------------------ Message: 3 Date: Wed, 26 Sep 2012 16:02:38 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: [Histonet] Question To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <5063271f.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII I was asked by a colleague if there is testing for "fibrinogen storage disease" that can be performed on fixed tissue. I told her that I did not know, but I would ask the experts. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax ------------------------------ Message: 4 Date: Wed, 26 Sep 2012 21:58:55 +0000 From: "Blair, Paula" <paula.bl...@cchmc.org> Subject: [Histonet] Ferret lungs To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <cc88f2de.1504%paula.bl...@cchmc.org> Content-Type: text/plain; charset="us-ascii" I am sectioning ferret lungs and am having problems with tearing and lines because the bronchioles and blood vessels are so thick , similar to trachea. Has anyone sectioned these animals and can give suggestions on soaking or anything that will help get a smooth section. ------------------------------ Message: 5 Date: Wed, 26 Sep 2012 17:44:48 -0500 From: Colleen Forster <cfors...@umn.edu> Subject: [Histonet] Come and visit the Legislative Committee in Vancouver! To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <50638560.6070...@umn.edu> Content-Type: text/plain; charset="iso-8859-1" Greetings to everyone on behalf of the Legislative Committee. I am formally inviting each of you to stop by our booth #625 while you are browsing about the product hall. We would love to meet you. This year we will have a couple different flag designs to look at or maybe you have another idea to present...come and vote and get your button! Our committee meets on Saturday September 29th at noon in room 208/209. Grab your lunch and join us. The more discussion we have the better! See you soon, Colleen Forster Legislative Chair. ------------------------------ Message: 6 Date: Thu, 27 Sep 2012 09:40:10 +0100 From: Debbie Faichney <d.a.faich...@stir.ac.uk> Subject: RE: [Histonet] softening agent for tissues embedded in paraffin blocks To: Jose Luis Palazon Fernandez <jluis.pala...@icman.csic.es>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8ed3f2ca5b78e142b8193376c57330f8fa2ea21...@exch2007.ad.stir.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Hello Jose, We routinely surface decalcify the trimmed/faced block for one hour with a commercial product (Rapid Decalcifier or RDC, Cellpath) which contains Hydrochloric acid. Works a treat! Alternatively we have been known to use a splash of laundry fabric softener in the soaking water for IHC sections. Which species of fish are you sectioning? This can make a huge difference to the timing in either solution. Best regards Debbie Faichney Histopathology Institute of Aquaculture University of Stirling Stirling, FK9 4LA UK .·´¯`·...¸><((((º>`·.¸¸.·´¯`·.¸.·´¯`·...,><((((º> .·´¯`·.......¸><((((º>`·....¸¸...·´¯`·.¸.·´¯`·...,..><((((º> -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jose Luis Palazon Fernandez Sent: 26 September 2012 10:56 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] softening agent for tissues embedded in paraffin blocks Dear List-members I'm trying to cut some fish muscle samples (along with skin and some bone tissues) embedded in paraffin (fixed in Bouin's fixative and decalcified with EDTA). I find it very difficult to cut these blocks and I would like to know, based in your experience, what tissue softening agent do you recomend to solve this problem. Many thanks in advance greetings, José Luis Dr. José Luis Palazón Fernández Instituto de Investigaciones Científicas Universidad de Oriente Boca del Rio-Isla Margarita-Venezuela Direccion actual: Instituto de Ciencias Marinas de Andalucia-CSIC Campus universitario Rio San Pedro, 11510, Puerto Real, Cádiz, España email: jluis.pala...@icman.csic.es; jose.pala...@ne.udo.edu.ve tlf: +34-956832612; fax: +34-956834701; cell: +34-600487100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- The University of Stirling is ranked in the top 50 in the world in The Times Higher Education 100 Under 50 table, which ranks the world's best 100 universities under 50 years old. The University of Stirling is a charity registered in Scotland, number SC 011159. ------------------------------ Message: 7 Date: Thu, 27 Sep 2012 07:05:05 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Ferret lungs To: "Blair, Paula" <paula.bl...@cchmc.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1348754705.63464.yahoomail...@web163102.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 You have to assure a perfect infiltration. Cartilage needs that. Also you will have to "play" with sectioning speed, type of blade (knife) and sectioning angle. René J. ________________________________ From: "Blair, Paula" <paula.bl...@cchmc.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Wednesday, September 26, 2012 5:58 PM Subject: [Histonet] Ferret lungs I am sectioning ferret lungs and am having problems with tearing and lines because the bronchioles and blood vessels are so thick , similar to trachea. Has anyone sectioned these animals and can give suggestions on soaking or anything that will help get a smooth section. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 106, Issue 34 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet