It has been my experience that tissues that remain in paraffin too long (like over a weekend) become brittle and hard. If we are embedding over 300 blocks, those blocks may remain in the embedding station for up to 6 hours - but I personally strongly recommend sticking to your SOP for processing. Besides, if your SOP says paraffin for 3 hours, leaving them longer is a violation of your SOP. Jackie O'
-----Original Message----- From: Demetria Ross <deshsmi...@gmail.com> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Thu, Sep 27, 2012 5:21 pm Subject: [Histonet] Paraffin and Tissue I'm curious to know how long can tissue stay on the machine in paraffin efore it becomes a problem I have left tissue stay in paraffin 30 min-2 ours before I take it off but not on a daily basis Thanks in advance ______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
