We run a control(s) first before using it on patient samples. We compare that slide to the same/similar control(s) run with the current lot. Our director reviews both slide(s) and if comparable or better, signs off on the new lot.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, October 01, 2012 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC new reagent lots I'm in a new position and have to get our IHC paperwork CAP ready. I'm not sure about something. When you put a new antibody dispenser (with a different lot # than previously used) on the IHC stainer, what QC needs to be done? Can the control run with a patient test serve as your QC, or does it need to be a separate slide done before you run any patient tests with that new lot #? Also, I know that CAP is no longer requiring negative controls to be run when using a biotin free detection system. Can we just stop running the negative controls, and reference the CAP change in our protocol, or do we have to perform some sort of validation? I appreciate any help. Deloris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet