My bad, I guess I misunderstood. I still like them clean, even if it seems unecessary.
Joelle Weaver MAOM, HTL (ASCP) QIHC > Subject: RE: [Histonet] RE: Metal molds > Date: Wed, 10 Oct 2012 12:18:48 -0600 > From: billodonn...@catholichealth.net > To: joellewea...@hotmail.com; brendal.fin...@medicalcenterclinic.com; > histonet@lists.utsouthwestern.edu > > Actually, the thread began because our VIP rotary valve got filled with > wax and required service. We clean daily in VIP, but wanted to know what > others were doing. Mostly, people are using the VIP, so we may need to > modify our regularity or change out cleaning solution more frequently. > > I do like where the thread has gone. I got my (chicken fryer) cast iron > skillet from my mother who got it from her mother. They were migrant > workers during the dust bowl, picking cotton in Texas and oranges in > California before settling in Arizona. I remember Mom frying chicken (or > the occasional rabbit) in it on Sunday mornings. Yes, I still use it, > but I have added several other cast iron pieces to the arsenal including > a bread pan! There will be pound cake in the oven tonight! > > William (Bill) O'Donnell, HT (ASCP) QIHC > Senior Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > SERENITY is not freedom from the storm, but peace amid the storm. > > Cultivate it in PRAYER! > > > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle > weaver > Sent: Wednesday, October 10, 2012 12:42 PM > To: brendal.fin...@medicalcenterclinic.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] RE: Metal molds > > > My impression of the intial thread was the general cleanliness and cross > contamination potential, not the fact that the blocks aren't easily > released. I have never had that issue. > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > Date: Wed, 10 Oct 2012 09:27:09 -0500 > > From: brendal.fin...@medicalcenterclinic.com > > To: histonet@lists.utsouthwestern.edu > > Subject: RE: [Histonet] RE: Metal molds > > > > > > We clean molds every day. My preferred method is heated water to melt > > > the paraffin off, then allow to cool. Peel the paraffin from the > > surface of the water, remove the molds from the water, then dunk them > > about 10 times in a mixture of alcohol and mold release. Allow to air > > > dry or dry in a low temp oven. > > > > > > In my experience, molds that aren't cleaned on a regular basis make it > > > very difficult to remove the embedded cassettes even if very, very > > cold. It's easier for me if my workspace and tools are clean and > > organized. > > > > > > -----Original message----- > > From: susan.wal...@hcahealthcare.com > > Date: Wed, 10 Oct 2012 01:00:50 -0500 > > To: joellewea...@hotmail.com, valerie.han...@parrishmed.com > > Subject: RE: [Histonet] RE: Metal molds > > > > > We put our molds in the VIP before running the cleaning cycle daily. > > Then we dip them in alcohol containing mold release..air dry and > > store. > > > > > > -----Original Message----- > > >From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle > > > weaver > > > Sent: Tuesday, October 09, 2012 3:27 PM > > > To: valerie.han...@parrishmed.com > > > Cc: histonet@lists.utsouthwestern.edu > > > Subject: RE: [Histonet] RE: Metal molds > > > > > > > > > I always cleaned them daily, either the very hot water, soapy water > > method, with water running over them in the sink with them on their > > sides so it passes over them, not upright so the water sits in them- > > then a rinse in alcohol and completely air dry. Or you can always do > > the clean cycle with the racks, running them through xylene, etc. They > > > come out very clean this way- used an old processor that was a backup > > for this most of the time. But I always did them daily, but also wiped > > > each one out with gauze if I used them twice in an embedding session ( > > > for more than one specimen in that large batch). Also I like metal, I > > hate those plasticones. If you keep the block face surface of the mold > > > warm-hot, and flatten before it turns completely white the specimen is > > > at the surface and you are able to see the edges easily without a lot > > of "facing". I think this saves time cutting through paraffin, and > > saves blades. Plus if the specimen is not flat enough, you see it > > right away and know if you must re-embed to get a complete, > > representative section, rather than after you have cut some > > superficial parts of some edges away and not others, only to have to > > re-embed anyhow. The other problems I see are when people are "afraid" > > of big molds- please if you are only taking one section, use one large > > > enough to leave a perimeter. Don't try to squeeze it into a medium > > mold, you are unlikely to need multiple sections on one slide and it > > is much easier to get flat and get a good section. Also please put > > enough paraffin on top, so that when it is cool the layer over the > > grooves in the cassette is not so thin that youcan clearly see the > > depressions. That little bit of paraffin is much cheaper than tech > > time in re-embedding and fussing with a block longer than you should. > > Not so much a big issue for many specimens, but anything hard/ dense, > > such as bone, cervix, uterus, leeps, ( you get the idea) it is not > > anchored enough without a good dose of paraffin, causing more chatter > > when you section, and maybe chipping out more frequently, or even the > > whole bottom surface to lift off the cassette. I guess I have some > > "pet peeves" with this topic, so thanks for letting me get that out! > > > > > > > > > > > > > > > Joelle Weaver MAOM, HTL (ASCP) QIHC > > > > From: valerie.han...@parrishmed.com > > > > To: billodonn...@catholichealth.net; > > histonet@lists.utsouthwestern.edu > > > > Date: Tue, 9 Oct 2012 10:51:01 -0400 > > > > CC: > > > > Subject: [Histonet] RE: Metal molds > > > > > > > > We clean our molds once a week. Soakthem in Xylene to remove > > paraffin, soak in 100% alcohol to remove xylene, rinse in running > > water, dry and spray with mold release solution. > > > > > > > > Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief > > > > Parrish Medical Center > > > > 951 N. Washington Ave. > > > > Titusville, Florida 32976 > > > > Phone:(321) 268-6333 ext. 7506 > > > > Fax: (321) 268-6149 > > > > valerie.han...@parrishmed.com > > > > > > > > > > > > -----Original Message----- > > > > From: histonet-boun...@lists.utsouthwestern.edu > > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > > O'Donnell, Bill > > > > Sent: Monday, October 08, 2012 4:32 PM > > > > To: histonet@lists.utsouthwestern.edu > > > > Subject: [Histonet] Metal molds > > > > > > > > > > > > OK folks, I know I should be smarter than this and I haven't seen > > discussion on itlately.... > > > > > > > > Are people cleaning their metal embedding molds after evey > > embedding session? > > > > > > > > If not, how often do you clean them? > > > > > > > > Do you clean them at all? > > > > > > > > If you clean them, how do you do it? > > > > > > > > Thanks > > > > > > > > Bill > > > > William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good > > Samaritan Hospital 10 East 31st Street Kearney, NE 68847 > > > > > > > > SERENITY is not freedom from the storm, but peace amid the storm. > > > > > > > > Cultivate it in PRAYER! > > > > > > > > > > > > > > > > > > > > This electronic mail and any attached documents are intended > > solely for the named addressee(s) and contain confidential > > information. 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