Hi All, Long time viewer, second time user.
I have Z-N stained sections, which show a "melted" homogenised appearance of the tissue with loss of nuclear detail compared to the H&E. Bancroft and Cook based procedure. I originally thought it was the microwave procedure, so I changed to the 60 degree oven incubation. Then at 37 degrees and then at RT. All Z-N stains show this loss of nuclear detail. Heat fixing is the same as per H&E. Acid Alcohol and Bluing Agent is the same as H&E. DCM is the same as H&E. Bringing to water is the same as H&E. Considering that it may be the particular 'brand' of stain used or the ingredients used in the stain. I used both dilute and conc Carbol Fuchsin (same brand). Same issue with nuclear detail loss. 1% Methylene Blue is the counterstain used. Water bath is at 'normal' temperature for sectioning. I have a 'feeling' that it is something in the Carbol Fuchsin that is causing this 'look'. My next option is to try another brand of Carbol Fuchsin. Any suggestions for improvement will be greatly appreciated. Regards Mitchell Wan InfinityPATH Pty Ltd M: 0418 745 750 P: 07 3123 8888 F: 07 3123 8889 E: mitchell....@infinitypath.com.au W: www.infinitypath.com.au 'There is a real magic in enthusiasm. It spells the difference between mediocrity and accomplishment.' - Norman Vincent Peale Sent from my Blackberry _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet