Hi there, Just take dog's appendix or lymphnode and titrate the antibody until it looks like the reference on www.cqpath.com
good luck! Marcel ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, October 22, 2012 7:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 107, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CD31 for FFPE IHC on Mouse (Amy Porter) 2. Renal biopsies and muscle biopsies (Vickroy, Jim) 3. Need help with spirochete control (Sheila Fonner) 4. RE: Renal biopsies and muscle biopsies (Lynette Pavelich) 5. RE: Need help with spirochete control (Lynette Pavelich) 6. RE: Renal biopsies and muscle biopsies (Morken, Timothy) 7. Dako cd3 and cd79a (Stuart Beaver) 8. RE: Dako cd3 and cd79a (Amy Porter) ---------------------------------------------------------------------- Message: 1 Date: Mon, 22 Oct 2012 08:24:10 -0400 From: "Amy Porter" <port...@msu.edu> Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse To: "'Patsy Ruegg'" <pru...@ihctech.net>, "'Mark Elliott'" <mark.elli...@hli.ubc.ca>, <histonet@lists.utsouthwestern.edu> Message-ID: <001801cdb050$241b8cf0$6c52a6d0$@edu> Content-Type: text/plain; charset="us-ascii" This antibody was the overwhelming consensus out of all the information I received. Thanks to all for the input!! Amy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Sunday, October 21, 2012 11:20 AM To: 'Mark Elliott'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse Rat anti mouse cd31 from Dionova is the way to go it is better than all the others. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: Friday, October 19, 2012 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD31 for FFPE IHC on Mouse Amy Can you please share the responses you got with the rest of us Thanks Mark Message: 8 Date: Thu, 18 Oct 2012 16:21:55 -0400 From: "Amy Porter" <port...@msu.edu> Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model To: "'Amy Porter'" <port...@msu.edu>,"'Histonet'" <histonet@lists.utsouthwestern.edu> Message-ID: <003101cdad6e$3792fc80$a6b8f580$@edu> Content-Type: text/plain;charset="US-ASCII" Thanks to all for responses......looks like most roads lead to once place which is spectacular!! Just what I needed. Amy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Porter Sent: Thursday, October 18, 2012 3:36 PM To: 'Histonet' Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model Anyone out there have CD31 working well on FFPE samples for Mouse Samples?? I know this might be a long shot, however I haven't looked for anything on this in quite awhile. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Patricia K. Senagore, M.D. - Consulting Pathologist Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 port...@msu.edu www.humanpathology.msu.edu ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 22 Oct 2012 07:58:12 -0500 From: "Vickroy, Jim" <vickroy....@mhsil.com> Subject: [Histonet] Renal biopsies and muscle biopsies To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bb0b9f1a8373f14fa2974e8cb24bf9cf049c6...@mmc-mail.ad.mhsil.com> Content-Type: text/plain; charset="us-ascii" I have been at the same hospital for over 34 years. I have seen pathologists come and go, and have seen changes in services like everyone has, some good and some bad. Since workflow changes demands that we do more with less, we are evaluating some of the extra things we do to see if some of these duties need to be done by other staff and not histotechs. In our hospital whenever a renal biopsy procedure is done in ultrasound they call the histology department and one of the staff go to the ultrasound and receive the tissue from the radiologist. The tech then brings the tissue to the gross room and the grossing staff separates and processes the specimen. In the old days ultrasound would page the renal pathologist and the renal pathologist would go get the specimens and then bring them to the gross lab, but it seems like many things this has now been relegated to the technical staff. We also pick up renal biopsies and muscle biopsies specimens from another hospital in the same town that sends us renal biopsies since we have the local renal pathologist. We do not routinely pick up any other specimens because we have a courier system, but the renal and sometimes muscle biopsy service still remains in the hands of the histology department. I would like to know how other hospitals handle transportation of specialized specimens such as renal biopsies and muscle biopsies. In the old days there was a purpose for a pathologist to be present since often the radiologist asked the pathologist to evaluate if he or she had enough adequate tissue, but today with better ultrasound methods rarely do the radiologists ask the techs to evaluate whether enough adequate tissue has been taken. I need some background information from others before I approach the powers to be to come up with an alternate plan so that the histotechs can spend more time in the lab and less time being a courier service for these biopsies. Of course an alternate way will have to include a method that is reliable and not threaten the patient results. Your thoughts? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 3 Date: Mon, 22 Oct 2012 06:17:35 -0700 (PDT) From: Sheila Fonner <fourfonn...@yahoo.com> Subject: [Histonet] Need help with spirochete control To: Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <1350911855.27329.yahoomail...@web31915.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello all, I am in need of some help with a spirochete protocol for the Leica Bond.? I am using a Biocare primary antibody.? I've tried ER1 for 20 min. with 15 min. Ab with no luck.? Does anyone do this, and do you think increasing the primary Ab time would help?? I've used this same Ab before on a different platform and had no problem.? Any help or advice would be greatly appreciated.? Thanks. Sheila, HT (ASCP) ------------------------------ Message: 4 Date: Mon, 22 Oct 2012 13:27:55 +0000 From: Lynette Pavelich <lpave...@hurleymc.com> Subject: [Histonet] RE: Renal biopsies and muscle biopsies To: "Vickroy, Jim" <vickroy....@mhsil.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <89f4666a496dc949a819ecc40e11c867bf564...@exchangemb1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" In our hospital, the radiology techs are responsible for bringing the renal biopsies up to the department (in saline) as soon as the procedure is obtained. They show the specimen to the attending pathologist, who signs off on the radiology record as sufficient. Then the histotechs divide the specimen for the EM, IF and NBF specimens. Our muscle/nerve biopsies are all done in-house up in OR and are sent directly to the lab in a dumbwaiter "fresh" on saline-soaked gauze where the histotechs then divide up the specimen according to protocol. The nurses are responsible for delivery to the histology department. hope this helps, Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Vickroy, Jim [vickroy....@mhsil.com] Sent: Monday, October 22, 2012 8:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Renal biopsies and muscle biopsies I have been at the same hospital for over 34 years. I have seen pathologists come and go, and have seen changes in services like everyone has, some good and some bad. Since workflow changes demands that we do more with less, we are evaluating some of the extra things we do to see if some of these duties need to be done by other staff and not histotechs. In our hospital whenever a renal biopsy procedure is done in ultrasound they call the histology department and one of the staff go to the ultrasound and receive the tissue from the radiologist. The tech then brings the tissue to the gross room and the grossing staff separates and processes the specimen. In the old days ultrasound would page the renal pathologist and the renal pathologist would go get the specimens and then bring them to the gross lab, but it seems like many things this has now been relegated to the technical staff. We also pick up renal biopsies and muscle biopsies specimens from another hospital in the same town that sends us renal biopsies since we have the local renal pathologist. We do not routinely pick up any other specimens because we have a courier system, but the renal and sometimes muscle biopsy service still remains in the hands of the histology department. I would like to know how other hospitals handle transportation of specialized specimens such as renal biopsies and muscle biopsies. In the old days there was a purpose for a pathologist to be present since often the radiologist asked the pathologist to evaluate if he or she had enough adequate tissue, but today with better ultrasound methods rarely do the radiologists ask the techs to evaluate whether enough adequate tissue has been taken. I need some background information from others before I approach the powers to be to come up with an alternate plan so that the histotechs can spend more time in the lab and less time being a courier service for these biopsies. Of course an alternate way will have to include a method that is reliable and not threaten the patient results. Your thoughts? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Mon, 22 Oct 2012 13:42:01 +0000 From: Lynette Pavelich <lpave...@hurleymc.com> Subject: RE: [Histonet] Need help with spirochete control To: Sheila Fonner <fourfonn...@yahoo.com>, Histonet <histonet@lists.utsouthwestern.edu> Message-ID: <89f4666a496dc949a819ecc40e11c867bf564...@exchangemb1.hmc.hurleymc.com> Content-Type: text/plain; charset="us-ascii" Hi Sheila, We also use the Leica Bond using the Biocare antibody. We have had success using the RED detection kit for the spirochete with this protocol: HIER 1 (6.0) for 20 minutes and 30 minutes in the antibody. hopes this helps, Lynette ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Sheila Fonner [fourfonn...@yahoo.com] Sent: Monday, October 22, 2012 9:17 AM To: Histonet Subject: [Histonet] Need help with spirochete control Hello all, I am in need of some help with a spirochete protocol for the Leica Bond. I am using a Biocare primary antibody. I've tried ER1 for 20 min. with 15 min. Ab with no luck. Does anyone do this, and do you think increasing the primary Ab time would help? I've used this same Ab before on a different platform and had no problem. Any help or advice would be greatly appreciated. Thanks. Sheila, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 22 Oct 2012 16:03:13 +0000 From: "Morken, Timothy" <timothy.mor...@ucsfmedctr.org> Subject: [Histonet] RE: Renal biopsies and muscle biopsies To: "Vickroy, Jim" <vickroy....@mhsil.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <761e2b5697f795489c8710bcc72141ff029...@ex07.net.ucsf.edu> Content-Type: text/plain; charset=us-ascii James, At our institution the techs do not go to kidney or muscle bx. The resident will deliver the fresh specimen to either directly to our lab or to grossing. Hospitals send them in fixative and transport media that we provide to them. My feeling is that if the techs are not there to check the adequacy of the specimen then there is no need for them to go. We used to do that routinely to ensure good material. These days we occasionally get an inadequate biopsy but considering we do over 700 renal and 200 muscle per year it is a very low percentage of the total and does not justify tech time to examine each one. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, October 22, 2012 5:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Renal biopsies and muscle biopsies I have been at the same hospital for over 34 years. I have seen pathologists come and go, and have seen changes in services like everyone has, some good and some bad. Since workflow changes demands that we do more with less, we are evaluating some of the extra things we do to see if some of these duties need to be done by other staff and not histotechs. In our hospital whenever a renal biopsy procedure is done in ultrasound they call the histology department and one of the staff go to the ultrasound and receive the tissue from the radiologist. The tech then brings the tissue to the gross room and the grossing staff separates and processes the specimen. In the old days ultrasound would page the renal pathologist and the renal pathologist would go get the specimens and then bring them to the gross lab, but it seems like many things this has now been relegated to the technical staff. We also pick up renal biopsies and muscle biopsies specimens from another hospital in the same town that sends us renal biopsies since we have the local renal pathologist. We do not routinely pick up any other specimens because we have a courier system, but the renal and sometimes muscle biopsy service still remains in the hands of the histology department. I would like to know how other hospitals handle transportation of specialized specimens such as renal biopsies and muscle biopsies. In the old days there was a purpose for a pathologist to be present since often the radiologist asked the pathologist to evaluate if he or she had enough adequate tissue, but today with better ultrasound methods rarely do the radiologists ask the techs to evaluate whether enough adequate tissue has been taken. I need some background information from others before I approach the powers to be to come up with an alternate plan so that the histotechs can spend more time in the lab and less time being a courier service for these biopsies. Of course an alternate way will have to include a method that is reliable and not threaten the patient results. Your thoughts? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Mon, 22 Oct 2012 17:22:32 +0100 From: Stuart Beaver <sbeaver0...@hotmail.co.uk> Subject: [Histonet] Dako cd3 and cd79a To: histonet@lists.utsouthwestern.edu Message-ID: <blu401-eas4590f55447af7fdc42f3b56e7...@phx.gbl> Content-Type: text/plain; charset="utf-8" Hi all, I am looking to for approximate dilution ranges for Dako cd3 and cd79a in canine lymph node samples for T and Bcell Lymphoma. I am using the Flex mini kit at the moment with high pH antigen retrieval. Regards Stuart ------------------------------ Message: 8 Date: Mon, 22 Oct 2012 12:52:55 -0400 From: "Amy Porter" <port...@msu.edu> Subject: RE: [Histonet] Dako cd3 and cd79a To: "'Stuart Beaver'" <sbeaver0...@hotmail.co.uk>, <histonet@lists.utsouthwestern.edu> Message-ID: <000601cdb075$aee88a70$0cb99f50$@edu> Content-Type: text/plain; charset="utf-8" Don't know what a Flex mini kit is - however we use Dako CD3 @ 1:100 for 60 minute / Heat retrieval steamer - ph 9.0 Tris EDTA / Vector secondaries and enzyme label HRP -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stuart Beaver Sent: Monday, October 22, 2012 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako cd3 and cd79a Hi all, I am looking to for approximate dilution ranges for Dako cd3 and cd79a in canine lymph node samples for T and Bcell Lymphoma. I am using the Flex mini kit at the moment with high pH antigen retrieval. Regards Stuart ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 107, Issue 28 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet