It depends upon what marker you're staining for. We fix our renal biopsies for C4d in acetone for 10".
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 1:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
