Hello,
As I wrote you some weeks ago, we started to use Technovit 9100 New for tissue embedding. You helped me to solve my previous problems. And I hope, you will help me to solve my present problems. Sorry, I can explain my problem as follow : The main problem is that we are having some troubles with the polymerization of Technovit 9100 New. The polymerization mixture does not want to become hard. We filled trial moulds with 3 ml of polymerization mixture. Moulds were vacuumed and sealed. We tried to perform the polymerization reaction at -30 degrees C, -20 degrees C, -4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails to polymerize. After a week, the polymerization mixture becomes gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem May be it is necessary to add more activator (dibenzoyl peroxide and N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions A and B in another proportion (not 9:1). Is it necessary to prepare the solution at 4 degrees too ? What should I do? Thank you in advance, _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet