You would have been better off leaving the tissues in formalin. You just would have to make sure HIER is correct (had you used your tissues for IHC procedures). Mice and rat tissues which are very lean, will suffer more in alcohol than in formalin. I would go back to formalin. René J.
From: Daniela Bodemer <daniela.bode...@mcri.edu.au> To: Histonet@lists.utsouthwestern.edu Sent: Monday, December 3, 2012 12:00 AM Subject: [Histonet] Fixation time Hi all, Our tissue processor has been shut down due to a contamination issue and now all the tissues (mice and rat pelves) collected prior to this happening have been sitting in 4% PFA. Some tissues more than a week, when we usually fix for 48 hours. Now we are transferring the tissues to 70% Ethanol and they will sit there until further notice. I am concerned about this process and what it will do to the tissue and would like your thoughts on this. Many thanks, DB Research Assistant ______________________________________________________________________ This email has been scanned by the Symantec Email Security.cloud service. For more information please visit http://www.symanteccloud.com ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet