Dear all, I am trying to detect cytokines in human pancreatic sections from diabetics. The blocks are at least 1y old, the sections are usually fresh. I am fixing the slides with 2% PFA and permeabilizing with 0.1% saponin, staining overnight in BSS-saponin with the primary, biotinylated antibody and subsequently tyramid signal amplification and fluorescein based visuatlisation. However, my results are very poor. I am mainly interested in these cytokines: IL1beta IL1r IL2 IL6 IL10 IL17 IL21 TNFalpha IFNgamma
Is there somebody out there who is experienced in detecting these cytokines, and could provide me with a comprehensive protocol? Also, who knows what the best positive controls might be? Thank you so much, Darius La Jolla, USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
