I have request to implement this stain for frozen muscle in OCT blocks. The pathologist is looking for demonstration of ragged red fibers, however it is my understanding (very limited) that this (in this circumstance) is a diagnostic stain and the tissue on hand that I will be using for development will be normal with no RRF present. I am unable to discern what type of tissue to use as a positive control, outside of muscle diseased specimen. Any suggestions and/or working methods would be greatly appreciated.
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