Kimura, The answer to your question is YES. Both Tween 20 & Triton 100 are non-ionic detergents. Tween 20 is not as strong/effective as Triton 100, but you can use higher percentage Tw20 or prolong the permeabilization timing. Best! Madeleine Huey, BS, MT, HTL & QIHC (ASCP) madelein...@elcaminohospital.org
On Fri, Dec 28, 2012 at 10:01 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Subject: [Histonet] In permeabilization, can we use Tween-20 instead > of Triton-100? > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > <cachjr3+kz7a_iazkws_bfps4fhf05vngshfyuykslderrnh...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Dear Histonetters > > Hi. > Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL). > The primary antibody is for detecting some intracellular > antigen(antigen in cytoplasm). > So I think permeabilization process may be necessary after fixation by 4% PFA. > Does anyone know whether we can use Tween-20 in place of Triton-100 > for permeabilization? > And can we use same protocol in immunostaining of BAL cells as in > immunostaining for cell line? > > Thanks in advance. > > H Kimura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
