To everyone in histoland that is familiar with IHC, I am a new histologist, unfamiliar with many techniques in IHC. Here in my lab, we section devices that contain cells, membrane and scaffolding. Unfortunately, due to the scaffolding, the devices do not hold up well when embedded in paraffin. Likewise, the other plastics I am able to cut nice sections with do not take well to immuno staining. Does anyone have any suggestions for a specific type of plastic resin that works well with IHC? (I could try something other than plastic too). For the time being, I use Dorn & Hart acrylosin soft... but the results are medicore. We will be doing a lot more immunohistochemistry in the near future, and I'm looking to experiment with different resins to find one that works well. Any help is appreciated!
Thanks for your time! Sincerely, Allyse Mazzarelli Histologist, Neurotech USA Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet