We use xylene free processing + microwave Formalin, 100% ethanol, isopropanol (clearant) and paraffin and have been doing so for 4 years
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: January 9, 2013 12:16 PM To: Jones, Laura; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Free Processing? The Xpress microwave tx processors are xylene free - perhaps they have one. I am pretty certain there still needs to be something that is misable with alcohol and paraffin, such as mineral oil. I believe this can be added to one or two of the paraffin steps, but I am just venturing a guess. Most processors would still require a xylene (or substitute) cleaning cycle to remove paraffin. I will be following this thread with interest - Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, January 09, 2013 1:38 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Free Processing? I would appreciate hearing all of your expert opinions on processing tissue without the use of xylene or any type of xylene substitute. Our processor was down recently, and a friendly local lab processed our tissue this way for us. Of course, the Pathologists loved it. The process was formalin, followed by graduated percentages of only isopropyl alcohol, and then paraffin. What are your feelings concerning immunos? Overall cost? Any other thoughts? Thanks in advance! ________________________________ Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet