Dear all,
I work in an histopathology department and we intend to do immunohistochemistry with an antibody generated in human on human tissues. So far, we don't have any experience with that. So I was wondering if somebody would have some feedback to prevent strong background generated by this human antibody on human tissue. We are planning in a first place to decrease the concentration of the secondary antibody Donkey@human which is recommended at 1/200 by Jackson, using it at 1/500 for example. But if anybody would have some experience with this kind of experiments, their tricks would be more than welcome. Best Regards, Carole Chotard Carole Chotard, Ph.D. Pharmacology Research Scientist Molecular Pharmacology and Pathophysiology Department IDRS 125 Chemin de Ronde 78290 Croissy-sur-Seine France Phone : +33 1 55 72 29 48 Fax : +33 1 55 72 25 96 carole.chot...@fr.netgrs.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet