Good advice given. Biotinylated primaries to be followed by stAvidin-488 or stABCpx-DAB, for eg. Or, if biotinylated primary gives good result with above but, at low dilution factor, use tyramide amplification?
Human- on- human can be just fine, depending on what proteins you want to demonstrate and, in what tissue. What tissue are you working on? What tissue prep ( Pwax/frozen)? You using patients' serum containing auto-antibodies? Or, do you have a human that you are injecting human Ags into, then bleeding that person so you can harvest Abs? Scary! NB: You suggested diluting out your secondary...not good as you will just lower your specific signal as well, imho. Curious Carl Carl Hobbs Histology Manager Wolfson CARD School of Biomedical Sciences Kings College London Guys Campus SE1 1UL Tel: 020 78486813 Fax: 020 78486816 020 78486813 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
