Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining.
>From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
