Michael,
Do you know or can you get the program they used to process the brain tissue? Also are they whole or slices of brain (at what thciknees)? Pam Marcum ----- Original Message ----- From: "Michael J. Lyon, Ph.D." <[email protected]> To: "Histonet" <[email protected]> Sent: Thursday, January 17, 2013 10:43:02 AM Subject: [Histonet] Paraffin sections mouse brain Hi everyone: While I have sectioned a lot of different stuff, I have not done mouse brain before and I am having considerable difficulty. The brains are fixed and were embedded by our pathology department on their automated system. When cutting, the paraffin cuts nicely but the brain tissue shreds as if the infiltration was insufficient. I have re-embedded one, just melted and new paraffin then vacuum oven for 2 hours. The results were the same. I don't know if it would help to re-embed going back to EtOH. Also, from my distant past I recall that when we were paraffin sectioning whole human larynges, that the face of the block was soaked by placing a wet gauze pad on it. I know this help but don't remember what the solution was on the pad. Any help would be appreciated. Thanks Michael J. Lyon, PhD Otolaryngology Research Labs SUNY Upstate Medical University 750 East Adams Street Syracuse, NY 13210 Voice: 315-464-7253 Fax: 315-464-5572 _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
