Pedro -
Since horse eyes are so large and dense, if possible the eye should be fenestrated to allow the fixative to penetrate the interior. Injection is also and option. Davidson's is the preferred fixative, as opposed to Bouin's, as it is fast penetrating and less hazardous. After initial fixation with Davidson's (or modified Davidson's), place the eye in 70% alcohol overnight. There is no need to place the eye in formalin after the Davidson's fixation. Cheryl Crowder, BA, HTL(ASCP) Crowder Histology Consulting 4952 Alvin Dark Ave. Baton Rouge, LA 70820 (225) 772-2865 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet