-----Original Message----- From: Hannen, Valerie Sent: Tuesday, January 29, 2013 5:46 AM To: 'Bob Richmond' Subject: RE: [Histonet] Re: Thrombin for cell block preparations
We get some Thromboplastin from the Hemo. Deparment and get Plasma from Blood Bank, when we need to make a cell block we add an equal amount of drops from both and let the specimen clot up. Hope this helps!! Valerie Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Monday, January 28, 2013 5:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Thrombin for cell block preparations Laurie Best, Histology Supervisor at Sunrise Medical Labs, Hicksville NY asks: >>I am trying to purchase thrombin for cell block preparation, and have >>been running into dead ends. Can someone give me their vendor >>information?<< I know about using thrombin to clot plasma to form cytopathologists' cell blocks, but have never seen it done, and don't know a source. In the many labs I've worked in, I've found that cell block methods and results vary greatly from lab to lab, from labs that don't do them at all, to labs that do them routinely on pleural and peritoneal fluids with uniformly good results. If I were setting up a procedure, I'd probably try the commercial agar preparation Histogel. Cell blocks are a necessary cytologic preparation, the more so as the use of immunohistochemistry increases. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ===================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet