Yes we monitor the temp and humidity. Retrieval is made fresh for every run. We run the PT for the p16 in a water bath since we only run a few slides at a time, but the water bath temp in also monitored. I should say that there is some staining in these slides just not what the control usually looks like. If we repeat the slides they do stain correctly. There is no difference in the slide prep or the staining procedure. Any ideas. Cindy
Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com -----Original Message----- From: Ross Benik [mailto:r...@premierlab.com] Sent: Wednesday, February 06, 2013 1:26 PM To: Cynthia Pyse Subject: RE: [Histonet] p16 Do you monitor the temperature and humidity inside the Link48? How often do you prepare your retrieval solution/are you using the PT mods? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Wednesday, February 06, 2013 11:11 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Happy Wednesday Everyone Has anyone been having consistency problems with their p16 staining since MTM has been taken over by Ventana? One day the procedure works another day it doesn't. Same procedure, no variation in the temperature on pretreatment, run on the Dako 48, with no errors. Every other IHC works consistently but the p16, anyone have any thoughts? I do make the DAB fresh daily as suggested in the instructions, it is the same kit that has been running. The only thing I can think of is we do sometimes run the p16 twice a day without making fresh DAB, but the instructions say it can be used within a 24 hour period. This problem is driving me crazy! It just doesn't make any sense. Any help would be appreciated. Thanks in advance. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet