Hi Colleen, Although I was not able to locate the specific reference you listed, the two stains that come to mind are Lendrum's Carbol Chromotrope and Congo Red. While they are not *really* specific for eosinophils, they do stain eosinophils well. It is fairly easy to differentiate an eosinophil from amyloid in Congo Red. I am including a link to Eastwood & Cole's Congo Red because the procedure specifically mentions eosinophils, but I have used Highman's (the more common version) to identify them. Of the two the Congo Red techniques seem to work better which is great since they are a bit easier (& less messy) than the carbol chromotrope.
Here are a couple of references for both: http://stainsfile.info/StainsFile/stain/cell/lendrum-carbolchromotrope.htm and http://stainsfile.info/StainsFile/stain/amyloid/congoeastwood.htm Good Luck, Amos On Tue, Feb 12, 2013 at 1:00 PM, <histonet-requ...@lists.utsouthwestern.edu>wrote: > Message: 6 > Date: Mon, 11 Feb 2013 16:36:29 -0600 > From: Colleen Forster <cfors...@umn.edu> > Subject: [Histonet] Stain to ID eosinophils specifically > To: Histonet <histonet@lists.utsouthwestern.edu> > Message-ID: <5119726d.7000...@umn.edu> > Content-Type: text/plain; charset="iso-8859-1" > > Does anyone have access to this reference for me? I have a person looking > to ID eosinophils in fat samples. > I am looking for a stain that will make finding them easier....this > reference was mentioned....any other suggestions? These are FFPE samples. > > Suggested Ref ex. LIllie 4th Ed. pp750. ref.Am.J.Clin.Path.55:283,1971.) > > Thanks in advance... > > Colleen L. Forster > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet