I fix in NBF at pH7 exactly and decalcify with EDTA René J. From: Clare Thornton <cthorn...@dahlchase.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Thursday, February 14, 2013 9:22 AM Subject: [Histonet] bone marrow specimens
What fixative and decal solution is everyone using for bone marrow specimens which will have subsequent IHC and Kappa/Lambda ISH staining? We are currently using B+ fixative and Decal A (formic acid and formaldehyde). Our pathologists demand a quick turn around time, and are willing to sacrifice some quality for this, but we are having issues with our Kappa/Lambda ISH stains not highlighting all the plasma cells. The staining tends to be stronger on the outer edge of the tissue, and weakens or disappears entirely towards the middle, so I'm thinking the fixation and/or decal is the problem. We run our K/L ISH using Ventana instrumentation and reagents. Thank you in advance! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
