"Standard" protocol is a very "encompassing" word and implies that it is "standard" for all labs and there is no such standard. Each lab has its own (although sometimes adhering to some general consensus) "standard" protocol, so probably that pharmaceutical company is referring to their "standard" protocol. Why don't you ask them to send you their "standard" protocol. Generally speaking your section will be floated and then the slide is tilted to assure the water totally draining from behind the section and are oven dried after and that is all. I do not see any advantage in dewaxing (=dipped for 3 minutes in xylene) and dried again. It even may be damaging to the section being exposed "naked" (without paraffin) to air. There has to be some error in those instructions by somebody not quite familiar with histology, as is usually the norm when dealing with pharmaceutical investigators. Ask them about these "non histological" instructions. René J.
From: Histopatty <histopa...@aol.com> To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 14, 2013 11:02 AM Subject: [Histonet] standard protocol We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes." I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt? Patricia Eneff for Kay Pierce Please respond to sharon.pie...@hcahealthcare.com if possilbe. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
