We see better results with FISH-slides baked over night. This is a phenomenon called aging, known from cytogenetics. It seems, that the time of air-drying and heat leads to a better access of the probes to the dna. Therefor we let them also in the oven over the weekend. Gudrun
-----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Houston, Ronald Gesendet: Donnerstag, 14. Februar 2013 20:08 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH baking and dewaxing Can someone please explain why paraffin-embedded slides for FISH need to be baked for so long and have extensive dewaxing in xylenes and subsequent alcohol pretreatment? Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 changes of xylene 15 min each, and 100% ethanol 15 minutes each x2 before air-drying. I have asked them why so long and get the standard response -- "that's the way we always do it"! Thanks Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org<mailto:ronald.houston@nationwidechild rens.org> www.NationwideChildrens.org<http://www.nationwidechildrens.org/> "One person with passion is better than forty people merely interested." ~ E.M. Forster _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
