Billie, We use Dianova's antibody and purchase it from Fisher. We've performed this stain for a little over six months and seem to be having success with the following protocol: Dianova's IDH1 R132H Antibody, Diluted 1:100 Ventana Benchmark XT, iView Detection Standard CC1 37 degrees C 32 minute antibody incubation A/B Block I hope this helps! Roger Heyna Maywood, IL
>>> "Zimmerman, Billie" <bzimm...@gru.edu> 2/22/2013 3:20 PM >>> Does anyone perform this antibody?? We can't get it to work on the Ventana Ultra. PhenoPath in Seattle performs it on the Dako autostainer using a small size polymer detection kit. I would appreciate any input. Thanks, Billie Zimmerman Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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