We've had one tech complaining of streaks when cutting, across the paraffin. It's noticeable when cutting, although not clearly noticeable microscopically. This tech has tried everything, tightening and re-tightening of microtome parts, trying other people's chucks or blade holders, new blades, new lots of blades...everything we can think of. It doesn't appear to be the microtome. Last week, I noticed the same streaking occuring on my sections. No one else (we have 9 microtomes total) has complained of this issue. Could this be a paraffin problem? The streaks look like when you might have a bit of paraffin debris on your blade and cut a new section; however, this is occuring with brand new blades, almost every block. Any ideas? Thank you.
Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet