We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after alk phos reagent prior to substrate; still happening on some slides...anyone else have this experience or type of problem? Any experiences or suggestions are appreciated. Just can't figure out what is causing it..
Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory port...@msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet