It's better not to assume that a stain developed for smears will work on tissue sections without modification. The Gram stain is an obvious case in point.
Bob Richmond Samurai Pathologist Maryville TN ********************************************* On Wed, Feb 27, 2013 at 9:20 PM, Tony Reilly <tony_rei...@health.qld.gov.au> wrote: > It is true that I have never used it on tissue sections as I have usually > performed it on smears for the Microbiology department. However some of > the specimens stained were formalin fixed and stained well while others were > PVA fixed. > > regards > Tony > > > > > Tony Reilly B.App.Sc. , M.Sc. > > Chief Scientist, Anatomical Pathology > > Pathology Queensland-PA Laboratory > > ________________________________________________ > Health Services Support Agency | Department of Health > > > > Level 1, Building 15,Princess Alexandra Hospital > > Ipswich Road,WOOLLOONGABBA Qld 4102 > Ph: 07 3176 2412 > Mob: 0402 139411 > > Fax: 07 3176 2930 > > Email: tony_rei...@health.qld.gov.au > > Web: www.health.qld.gov.au/qhcss/ > > > > > > > >>>> Bob Richmond <rsrichm...@gmail.com> 2/28/2013 12:12 pm >>> > > You're referring I suppose to the "Gomori-Wheatley" modification of > the one step trichrome, used for staining intestinal amoebae. Don't > know if it will work on tissue sections. It has quite a number of > variants. > > Once again, I'd advise consulting an eye pathologist. > > Bob Richmond > Samurai Pathologist > Maryville TN > ***************** > On Wed, Feb 27, 2013 at 8:58 PM, Tony Reilly > <tony_rei...@health.qld.gov.au> wrote: >> Hi Matt >> >> The Heidenhain's stain is good but if you want one that is prettier try >> Gomori's one step trichrome. I have only ever used it for intestinal >> protozoa but I think it would work for all. >> >> regards >> Tony >> >> Tony Reilly B.App.Sc. , M.Sc. >> >> Chief Scientist, Anatomical Pathology >> >> Pathology Queensland-PA Laboratory >> >> ________________________________________________ >> Health Services Support Agency | Department of Health >> >> Level 1, Building 15,Princess Alexandra Hospital >> >> Ipswich Road,WOOLLOONGABBA Qld 4102 >> Ph: 07 3176 2412 >> Mob: 0402 139411 >> >> Fax: 07 3176 2930 >> >> Email: tony_rei...@health.qld.gov.au >> >> Web: www.health.qld.gov.au/qhcss/ >> >>>>> Bob Richmond <rsrichm...@gmail.com> 2/28/2013 4:19 am >>> >> >> Matt Brooks asks: >>One of our Neuropath docs is inquiring about a >> special stain for unencased >> [amoebae] in cornea biopsies. I did a search and Gridley's method was >> the best option that appreaded [sic]. Is there someone that could >> either verify this stain will work on this organism or let me know >> what you stain you are using?<< and René Buesa suggests Heidenhain's >> iron hematoxylin. >> >> Corneal ulcers are caused by Acanthamoeba and related species, rather >> than by Entamoeba histolytica. Google >>amoeba corneal stain<< - some >> references suggest a fluorescent stain such as calcofluor white, if >> you have access to a fluorescence microscope. >> >> Iron hematoxylins provide exquisite nuclear detail, but are >> challenging to set up for the first time (I've done it), and are not >> specific. >> >> Periodic acid Schiff (PAS) is often used to demonstrate the glycogen >> in Entamoeba histolytica trophozoites, but I don't know if the other >> pathogenic amoebae contain glycogen. >> >> My advice would be to ask one of the academic eye pathologists. My >> choice would be Dr. Hans Grossniklaus at Emory, but there are a good >> many more. And remember you'll need to find a control slide. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN > > ******************************************************************************** > > This email, including any attachments sent with it, is confidential and for > the sole use of the intended recipient(s). This confidentiality is not > waived or lost, if you receive it and you are not the intended recipient(s), > or if it is transmitted/received in error. > > Any unauthorised use, alteration, disclosure, distribution or review of this > email is strictly prohibited. The information contained in this email, > including any attachment sent with it, may be subject to a statutory duty of > confidentiality if it relates to health service matters. > > If you are not the intended recipient(s), or if you have received this email > in error, you are asked to immediately notify the sender by telephone > collect on Australia +61 1800 198 175 or by return email. 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