Our alcohols that cannot be recycled (contaminated with xylene) are combined with other flammable waste and dealt with by an external company.
L.White -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, February 26, 2013 12:36 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Maxvison anti-Rabbit HRP Antibody Substitution (Suresch, Donna L.) 2. RE: Maxvison anti-Rabbit HRP Antibody Substitution (Sarah Dysart) 3. CAP checklist question (Hannen, Valerie) 4. Ventana Labels (Matthew Roark) 5. Re: Ventana Labels (Mark Tarango) 6. RE: Ventana Labels (Bitting, Angela K.) 7. IgG4 (Susan Foreman) 8. Streaking when Cutting (nmhi...@comcast.net) 9. Waste Alcohol (Scott, Allison D) 10. RE: Waste Alcohol (Rathborne, Toni) 11. RE: Waste Alcohol (Horn, Hazel V) 12. RE: Waste Alcohol (Mike Pence) 13. Re: Waste Alcohol (Rene J Buesa) 14. Dako autostainer service (Karla J. C.) 15. RE: CAP checklist question (susan.wal...@hcahealthcare.com) 16. Question for Alkaline Phosphatase / Biotin System Users (Amy Porter) 17. RE: Waste Alcohol (Horn, Hazel V) 18. RE: Waste Alcohol (Podawiltz, Thomas) 19. Re: Waste Alcohol (Brendal Finlay) 20. streaks (Sheila Fonner) ---------------------------------------------------------------------- Message: 1 Date: Mon, 25 Feb 2013 13:07:02 -0500 From: "Suresch, Donna L." <donna_sure...@merck.com> Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <2c9a1d9608959940943f357e0a470ff8b2a9d0b...@usctmxp51005.merck.com> Content-Type: text/plain; charset="us-ascii" Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------ Message: 2 Date: Mon, 25 Feb 2013 18:15:20 +0000 From: Sarah Dysart <sdys...@mirnarx.com> Subject: [Histonet] RE: Maxvison anti-Rabbit HRP Antibody Substitution To: "Suresch, Donna L." <donna_sure...@merck.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8a70a9b2ecdd084dacfe6c59fcf86d5048505...@bl2prd0711mb434.namprd07.prod.outl ook.com> Content-Type: text/plain; charset="us-ascii" The Biocare polymers (MACH2 series) are excellent!! Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suresch, Donna L. Sent: Monday, February 25, 2013 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Maxvison anti-Rabbit HRP Antibody Substitution Hello All, I have been using Maxvision anti-Rabbit HRP secondary antibody with excellent results. The product has been discontinued. Has anyone found a good substitution for this antibody? Thank you. Donna L. Suresch Senior Imaging Research Scientist Merck Research Laboratories Department of Imaging - West Point Campus Mail Stop: WP44K Office: WP44-H129 770 Sumneytown Pike PO Box 4 West Point, PA 19486-0004 Phone: 215-652-7349 Fax: 215-993-6803 Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 25 Feb 2013 13:21:16 -0500 From: "Hannen, Valerie" <valerie.han...@parrishmed.com> Subject: [Histonet] CAP checklist question To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7BEC@isexstore03> Content-Type: text/plain; charset="us-ascii" Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com =================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" =================== ------------------------------ Message: 4 Date: Mon, 25 Feb 2013 13:33:55 -0600 From: "Matthew Roark" <mro...@sfmc.net> Subject: [Histonet] Ventana Labels To: <histonet@lists.utsouthwestern.edu> Message-ID: <002101ce138f$0cf92a30$26eb7e90$@net> Content-Type: text/plain; charset="us-ascii" Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mro...@sfmc.net http://www.sfmc.net ------------------------------ Message: 5 Date: Mon, 25 Feb 2013 11:43:38 -0800 From: Mark Tarango <marktara...@gmail.com> Subject: Re: [Histonet] Ventana Labels To: Matthew Roark <mro...@sfmc.net> Cc: histonet@lists.utsouthwestern.edu Message-ID: <CACJwj1K6NiDPJX8m=qakbqqpmyhb+paaeaeyjqemc_prvju...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi Matthew, We use them and they work fine. They are much cheaper too. We've had them for about 3 years. I initially had some concerns about the DAB being absorbed and staining the labels on some cases. Our safety person told me that it's not a safety concern and it's safe to touch the label after it's stained brown by DAB. This doesn't happen on every slide but is a random thing. It might have something to do with how well it was affixed to the slide. The labels don't affect the staining in my experience. Mark On Mon, Feb 25, 2013 at 11:33 AM, Matthew Roark <mro...@sfmc.net> wrote: > Hello all, > > I am getting ready to try some slide labels as an alternative to the > Ventana labels. I was wondering if anyone else is using them or have > tried them in the past. > > They do not have that plastic fold over flap and are suppose to be > quite economical, though I have not got a quote yet. > > They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) > - Mercedes Medical Supply sent me the trial roll. > > Thanks! > > > Matthew Roark- HT/HTL(ASCP)CM > Histology Specialist > Saint Francis Medical Center > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > 573-331-5267 > mro...@sfmc.net > http://www.sfmc.net > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Mon, 25 Feb 2013 19:55:59 +0000 From: "Bitting, Angela K." <akbitt...@geisinger.edu> Subject: RE: [Histonet] Ventana Labels To: Matthew Roark <mro...@sfmc.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <77f52efab8b1694b885e277c48fcd0f632850...@ghsexmbx4w8k1v.geisinger.edu> Content-Type: text/plain; charset="us-ascii" I just tried them Friday. They worked fine but the label picked up counterstain a bit. However, depending on the cost per label, it may be worth the trouble. I'm waiting for a quote. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Matthew Roark Sent: Monday, February 25, 2013 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Labels Hello all, I am getting ready to try some slide labels as an alternative to the Ventana labels. I was wondering if anyone else is using them or have tried them in the past. They do not have that plastic fold over flap and are suppose to be quite economical, though I have not got a quote yet. They are called FloProTek (http://www.easternlabsvc.com/floprotek.php) - Mercedes Medical Supply sent me the trial roll. Thanks! Matthew Roark- HT/HTL(ASCP)CM Histology Specialist Saint Francis Medical Center 211 Saint Francis Drive Cape Girardeau, MO 63703 573-331-5267 mro...@sfmc.net http://www.sfmc.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ------------------------------------------------------------------------- This message was secured by ZixCorp(R). ------------------------------ Message: 7 Date: Mon, 25 Feb 2013 14:57:25 -0500 From: "Susan Foreman" <sfore...@labpath.com> Subject: [Histonet] IgG4 To: <histonet@lists.utsouthwestern.edu> Message-ID: <002e01ce1392$554fa900$ffeefb00$@com> Content-Type: text/plain; charset="us-ascii" Has anyone worked up IgG4 on the Ventana Benchmark Ultra? What vendor? How about running it on fresh frozen sections? FITC? Vendor? Many Thanks for your input, Susan ------------------------------ Message: 8 Date: Mon, 25 Feb 2013 20:09:08 +0000 (UTC) From: nmhi...@comcast.net Subject: [Histonet] Streaking when Cutting To: HISTONET <histonet@lists.utsouthwestern.edu> Message-ID: <1822368949.1502216.1361822948867.javamail.r...@sz0075a.emeryville.ca.mail.c omcast.net> Content-Type: text/plain; charset=utf-8 I've been quietly observing from the sidelines, but I just can't pass this one up.B One should never streak when cutting - it's distracting to fellow techs.B Okay - back to my nap. ------------------------------ Message: 9 Date: Mon, 25 Feb 2013 20:27:55 +0000 From: "Scott, Allison D" <allison.sc...@harrishealth.org> Subject: [Histonet] Waste Alcohol To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fa607dc3d1ed7c46a9546820a3eb877f0a1e1...@lbmsg02.hchd.local> Content-Type: text/plain; charset="us-ascii" Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ------------------------------ Message: 10 Date: Mon, 25 Feb 2013 20:33:27 +0000 From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> Subject: [Histonet] RE: Waste Alcohol To: "'Scott, Allison D'" <allison.sc...@harrishealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <3ad061fe740d464fac7bf6b5cfb75707599df...@smcmail02.somerset-healthcare.com> Content-Type: text/plain; charset="us-ascii" We have been disposing of our alcohols like this for years. This would include alcoholic eosin too. We are allowed to mix the waste xylene with the alcohols because they are the same classification, and treated the same way. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 3:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 25 Feb 2013 15:02:10 -0600 From: "Horn, Hazel V" <hor...@archildrens.org> Subject: [Histonet] RE: Waste Alcohol To: "'Scott, Allison D'" <allison.sc...@harrishealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <25a4de08332b19499904459f00aaacb719be135...@evs1.archildrens.org> Content-Type: text/plain; charset="us-ascii" Our xylene and alcohol go into a waste drum to be disposed of. We do recycle both but of course we still have waste to dispose of. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Monday, February 25, 2013 2:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 12 Date: Mon, 25 Feb 2013 21:18:12 +0000 From: Mike Pence <mpe...@grhs.net> Subject: [Histonet] RE: Waste Alcohol To: "Scott, Allison D" <allison.sc...@harrishealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <d6df96c438fb8d42b04b2345a9452cf5a...@mail-mb01.grhs.net> Content-Type: text/plain; charset="us-ascii" You should be recycling both xylene and alcohol. Cost savings compared to disposal cost will pay for a recycler in 3-5 years. ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Scott, Allison D [allison.sc...@harrishealth.org] Sent: Monday, February 25, 2013 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 25 Feb 2013 13:53:22 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Waste Alcohol To: "Scott, Allison D" <allison.sc...@harrishealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1361829202.27805.yahoomail...@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Reni J. From: "Scott, Allison D" <allison.sc...@harrishealth.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 25 Feb 2013 13:47:34 -0800 From: "Karla J. C." <ka...@dermatopathologynorthwest.com> Subject: [Histonet] Dako autostainer service To: <histonet@lists.utsouthwestern.edu> Message-ID: <000f01ce13a1$b8de93a0$2a9bbae0$@dermatopathologynorthwest.com> Content-Type: text/plain; charset="us-ascii" Hi All, Help.Dako just recently informed us that they would no longer provide a Service contract for our immuno autostainer. Is anyone else experiencing this? Does anyone know of any other service people that may be able to work on this equipment (model 3400 year 2003)? We are located in Seattle. Thanks, Karla Carlmas Dermatopathology Northwest ------------------------------ Message: 15 Date: Tue, 26 Feb 2013 01:55:58 -0600 From: <susan.wal...@hcahealthcare.com> Subject: [Histonet] RE: CAP checklist question To: <valerie.han...@parrishmed.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <4bf03f5404ebde409af9232da74b9ded2dff10b...@fwdcwpmsgcms09.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We put in a procedure stating that should we change the schedule we will test a variety of tissue (10 different types) and have the pathologist assess the run. We have not changed a schedule in years but we seek to comply... -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Monday, February 25, 2013 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP checklist question Hi gang.. I am working on a self-inspection CAP checklist and came across the following: ANP.23120 Tissue Processor Tissue processing schedules are validated. Note: New tissue processing schedules must be validated against the standard laboratory processing schedule. Evidence of compliance: Written procedure for validation of new tissue processing schedules AND WC records documenting validation What do you all make of this?? We have not had any new processing schedules introduced into our lab in years. So, I really don't know how to proceed on this one. Any insite will be greatly appreciated. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com ============= "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ============= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 26 Feb 2013 09:14:28 -0500 From: "Amy Porter" <port...@msu.edu> Subject: [Histonet] Question for Alkaline Phosphatase / Biotin System Users To: <histonet@lists.utsouthwestern.edu> Message-ID: <000d01ce142b$96d73ac0$c485b040$@edu> Content-Type: text/plain; charset="us-ascii" We are using standard avidin / biotin staining with alkaline phosphatase labeling and sometimes, not every time and not every slide in the same run we get residual blotchy spots and streaking of the reaction on and off the tissue. We have increased to two rinses after primary and two rinses after alk phos reagent prior to substrate; still happening on some slides...anyone else have this experience or type of problem? Any experiences or suggestions are appreciated. Just can't figure out what is causing it.. Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory port...@msu.edu ------------------------------ Message: 17 Date: Tue, 26 Feb 2013 09:27:03 -0600 From: "Horn, Hazel V" <hor...@archildrens.org> Subject: RE: [Histonet] Waste Alcohol To: "'Rene J Buesa'" <rjbu...@yahoo.com>, "Scott, Allison D" <allison.sc...@harrishealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <25a4de08332b19499904459f00aaacb719be135...@evs1.archildrens.org> Content-Type: text/plain; charset="iso-8859-1" The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Reni J. From: "Scott, Allison D" <allison.sc...@harrishealth.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ------------------------------ Message: 18 Date: Tue, 26 Feb 2013 10:55:14 -0500 From: "Podawiltz, Thomas" <tpodawi...@lrgh.org> Subject: RE: [Histonet] Waste Alcohol To: "Horn, Hazel V" <hor...@archildrens.org>, 'Rene J Buesa' <rjbu...@yahoo.com>, "Scott, Allison D" <allison.sc...@harrishealth.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <38667e7fb77ecd4e91bfaeb8d986386324fcf3c...@lrghexvs1.practice.lrgh.org> Content-Type: text/plain; charset="iso-8859-1" Reagent Alcohol contains methanol, which is a U-listed EPA hazardous chemical and cannot be poured down the drain. Tom Podawiltz HT (ASCP) Histology Section Head/Laboratory Safety Officer. LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Tuesday, February 26, 2013 10:27 AM To: 'Rene J Buesa'; Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Waste Alcohol The ability to put alcohol down the drain is regulated by your wastewater utility. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, February 25, 2013 3:53 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Waste Alcohol I always dumped the ethanol down the drain. I never recycled it because it takes almost 3 times more time that recycling xylene (3 times the cost) and the recycled alcohol is seldom of good quality. When I stopped using xylene I had no more use (and exposure) when recycling. Reni J. From: "Scott, Allison D" <allison.sc...@harrishealth.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Monday, February 25, 2013 3:27 PM Subject: [Histonet] Waste Alcohol Hello to all in histoland. Is there anyone putting alcohol into a waste drum like for xylene. We have a company that takes away our xylene. Now the safety people are suggesting that we treat the alcohol the same way. We have always poured the alcohol down the drain. It seems very expensive. Your help in this matter will be greatly appreciated. Allison Scott HT(ASCP) Supervisor, Histology Lab LBJ Hospital Harris Health System Office: 713-566-5287 Lab: 713-566-5287 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** **************************************************************************** ********************** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ------------------------------ Message: 19 Date: Tue, 26 Feb 2013 09:56:12 -0600 From: "Brendal Finlay" <brendal.fin...@medicalcenterclinic.com> Subject: Re: [Histonet] Waste Alcohol To: "Rene J Buesa" <rjbu...@yahoo.com>, "Scott, Allison D" <allison.sc...@harrishealth.org>, histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Message-ID: <4fcdbb6eed8fb2fc1e5ea8e615ce0...@medicalcenterclinic.com> Content-Type: text/plain; charset="utf-8" YouB will probably want to contact your local or state office of environmental protection to find out what is required in your area.B B The EPA lists hazardous wastes in 40 CFR 261.30.B Even if your waste is not on this list, it may have hazardous characteristics such as: ignitability, corrosivity, reactivity, and toxicity characteristic.B Ignitability pertains to flashpoints less than 140 F and/or aqueous solutions with alcohol content greater than or equal to 24 percent.B B Florida's DEP has different regulations dependent upon the quantity of hazardous waste generated.B Some localities may require approval to dispose of alcohol down the drain.B If you don't have this approval, you have to dispose of the waste according toB your stateB andB federalregulations.B B To reduce the quantity of hazardous waste we generate, we began to recycle using CBG Biotech's distillation unit.B We have had great results with it.B While alcohol does take longer to recycle than xylene, our supply costs have been greatly reduced and we have worked this into our weekly schedule very well. B Some things to consider when using recycled alcohol: Test each finished alcohol run with a hydrometer to know what percentage it is.B Do not use recycled alcohol at the end of the stain line after eosin (it will wash the eosin out). Do not use recycled alcohol as the last alcohol before xylene on the processor. Finally, do not recycle the first alcohols on the stain line after xylene (at least on our recycler). This is disposed of in our flammable waste containers to be picked up. B Hereb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet