Hi,

you can find a pratical suggestion at your problem by watching the Step 9: 
"Expedite Large
Specimen Fixation"

on the guide:
"101 Steps to Better
Histology - a Practical Guide to Good Histology Practice"
You can download it from:
http://www.leicabiosystems.com/pathologyleaders/101-steps-to-better-histology-a-practical-guide-to-good-histology-practice/

I don't know if such procedure is suitable for the study of your specimen but 
take it just like a suggestion.

My Best.
Massimo Tosi
"A humble
Chemical Engineer who loves Histology"


________________________________
 Da: Giulia Zunino <giuli.zun...@gmail.com>
A: histonet@lists.utsouthwestern.edu 
Inviato: Mercoledì 13 Marzo 2013 11:31
Oggetto: [Histonet] Fixation
 
I would like to have some informations about fetal brain fixation..
In general, it is in use the fomalin, but during the cut it is possible to
see that in the deep layer the formalin doesn't arrive...
So, Could you give some suggestion to improve this technique?!

Thanks in advance

Best





Giulia Zunino, PhD Student

Laboratory of Molecular Neuropathology
Centre for Integrative Biology (CIBIO), University of Trento
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