Hello all,


My pathologist gave me a copy of "Immunofluorescence with Dual Microwave 
Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy 
Specimens" from the American Journal of Clinical Pathology.  He said that the 
same principle should work on skin and he would like to be able to do IF on 
fixed tissue in addition to our usual cryostat sections.  Has anyone else read 
the paper who might be willing to give me some basic advice to try working it 
out?  Is a microwave necessary (paper's method uses 2 different wattage 
settings) or is there a way to use HIER in waterbath or pressure cooker?



Thanks in advance,

Erin



Erin Martin, Histology Supervisor
UCSF  Dermatopathology Service
415-353-7248

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