I too use the DAKO Autostainer and have for many years. My default setting is
100 ul and drop zone in the middle of the slide, however, I adjust the volume
and the drop zone depending on how large the section is and where the section
is on the slide in order to conserve reagents.I don't notice any difference in
staining.
Mark
UBC-James Hogg Research Centre
Vancouver BC
Message: 7
Date: Thu, 21 Mar 2013 15:53:08 +0000
From: Tiana Baskin <tfount...@dsmanitoba.ca>
Subject: [Histonet] IHC Stainer Dispense volume
To: "'histonet@lists.utsouthwestern.edu'"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<f95330f10f33db4397216c22a290427fb...@reg1msmx0021.ad.wrha.mb.ca>
Content-Type: text/plain; charset="us-ascii"
I currently use a Dako Autostainer and we are dispensing 300uL of all reagents
to the slide(100uL per drop zone). I am just curious what others are dispensing
and if you've noticed any difference by changing the dispense volume in regards
to stain quality. Does anyone adjust for very large tissue sections (that cover
the majority of the slide)?
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