Hi Jian, I specialize in orthopedic histology, and I also live in Pittsburgh. I would be glad to discuss the details of your project and see if I can be of any assistance to you. Please contact me at your convenience.
Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-268-8275 (fax) seanmcbride...@me.com On Mar 21, 2013, at 7:26 PM, Yu, Jian wrote: > Dear all, > > We like to make paraffin embedded bone of mouse tibia and cut longitudinal > sections for H&E sections. > > Does anyone have a easy-to-follow protocol to prepare (fix and decal) the > bone before processing? We routinely fix soft tissue (ie. mouse GI tract and > liver) and send off to our pathology core for processing and embedding. > > Any tips on cutting the blocks is helpful too. These sections appear brittle. > > We are also interested in a practical protocol for making mouse BM smears and > staining to determine cellularity changes after radiation. > > We just started working with the bone. Any advice will be very helpful and > greatly appreciated. > > Thanks, Jian > ******************************************************************* > Jian Yu, Ph.D. > University of Pittsburgh Cancer Institute > Email: y...@upmc.edu > ******************************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
