Hi All-
We have previously used Llewellyn sirius red protocol from Stainsfile with no issues to stain paneth cells. Recently, the sections are looking more like an H and E stain or completely red rather than the expected light blue (nuclei) and red (paneth) stain that we are accustomed to. The powder is from Sigma (its 2 years old) and we make a fresh solution for use each time. Hopefully you can provide some insight regarding this recent problem we are having. Thanks, Lillian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
