It depends on the antigen. I know that sounds like a cop out but it is true. Review the literature covering the antigen you are looking for to see what has worked for others

Geoff


On 4/11/2013 6:19 AM, Ian R Bernard wrote:
Pretreatments are used to recover bonded antigen sites owing to formalin 
linkage.  What is the optimum or maximum fixation time for tissues that may 
require Immunohistochemistry staining?

IB
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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