Elizabeth, We are able to detect human cells in mice organs using MAB1273 anti-mitochondria antibody (1:50) from Millipore on FFPE sections after standard HIER retrieval. As a detection reagent we use MaxFluor 594 Mouse on Mouse IF detection kit (MaxVision, cat.No MF03),
Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron Sent: Thursday, April 11, 2013 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti-human nuclear antibody Just wondering if anyone has had any luck with Millipore anti-nuclear antibody MAB1281, and if so, what was your protocol? We are trying to detect human cells in mice. Thank you. Elizabeth M. Cameron, HT, QIHC (ASCP) Histology Supervisor The Jackson Laboratory Bar Harbor, Maine The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet