We had this happen to us before. We had a bad lot of blades! Josie Britton HT QIHC (ASCP) Cheshire Medical Center Keene, NH 03431
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Denise G Crowley Sent: Thursday, April 11, 2013 9:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] compression problems Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This involves all 4 of our microtomes to varying degrees and all types of tissue. The only commonality is the paraffin. The tissue itself does not compress, but the paraffin around it looks like horizontal stripes. We use Paraplast Xtra for both infiltrating and embedding. Any suggestions? Denise Crowley Hope Babette Tang Histology Facility Koch Center for Integrative Cancer Research Massachusetts Institute of Technology 500 Main St. 76-182 Cambridge MA 02139 617-258-8183 dencr...@mit.edu<mailto:dencr...@mit.edu> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet