Thanks to Deloris for taking the time to attend our GSH symposium and her crochet bikini was beautiful! Thanks also to Janet Hobbs for bringing her crochet bikini. The speakers as well as the vendors were wonderful. It was a packed house and a lot of fun. Who says learning can't be fun?! It was a Class Act and we're looking forward to next year. Thanks again to everyone that attended.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, April 16, 2013 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Blades (Hale, Meredith) 2. Question regarding appropriate use of histonet (Henry Chidgey) 3. Thermohistoscreen Cassettes (DeMint, Valerie S) 4. Bone, Biomaterials & Medical Device Implants Histology Event In Cambridge, MA - May 4th, 2013 (Jack Ratliff) 5. (no subject) (Karla Arrington) 6. Glucose Oxidase blocking method (Long, Denise) 7. Home based slide interpretation (Jennifer MacDonald) 8. Immunohistochemistry for Adipose tissue macrophages in humans (khadija irfan) 9. Re: Home based slide interpretation (Rene J Buesa) 10. Picrosirius red on cryosections (Laura Avogaro) 11. RE: Picrosirius red on cryosections (Elizabeth Chlipala) 12. RELIA Hot Job Alert 4-16-2013 Exciting Opportunities in NC.VA, GA, CA, CO, IL, TX and NM (Pam Barker) 13. SP and CGRP antibodies (Dessoye, Michael J) 14. GSH Symposium (Deloris Carter) 15. RE: GSH Symposium (Shirley A. Powell) ---------------------------------------------------------------------- Message: 1 Date: Mon, 15 Apr 2013 17:39:26 +0000 From: "Hale, Meredith" <mh...@miracals.com> Subject: [Histonet] Blades To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <0E828EC51C7CC445A51E53F81B64E8C721A8B5@s-irv-exchmb.PathologyPartners.intranet> Content-Type: text/plain; charset="us-ascii" I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.com<mailto:mh...@miracals.com<mailto:mh...@miracals.com%3cmailto:mh...@miracals.com>> ------------------------------ Message: 2 Date: Mon, 15 Apr 2013 12:48:46 -0500 From: "Henry Chidgey" <hchid...@earthlink.net> Subject: [Histonet] Question regarding appropriate use of histonet To: <histonet@lists.utsouthwestern.edu> Message-ID: <005801ce3a01$7c705d00$75511700$@net> Content-Type: text/plain; charset="us-ascii" Howdy Folks, As I am sure you can tell we are subscribers to histonet and appreciate the generosity of members in helping solve the occasional problem we encounter. We are in need of finding either an individual or lab in our area to perform mammal root sectioning and slide preparation for the forensic mammal aging business we operate located near Burnet, Texas in the Texas Hill Country. I would like to reach out to your subscriber base to see if anyone is interested in doing some part time work for us or know someone who might be. Please let me know if that would be an appropriate use of the site. Thanks, Henry Chidgey CoFounder See what folks are saying about us <http://www.deerage.com/testimonials.htm> Wildlife Analytical Laboratories www.DeerAge.com <http://www.deerage.com/> PO Box 295 1303 CR 118 B Burnet, Texas 78611 (512) 756-1989 (512) 663-9476 Cell Serving Wildlife Stewards, Ranchers & Hunters Worldwide Customer Service and Quality of Analysis are our Priority and Passion Facebook <http://www.facebook.com/DeerAge> Eagle Soybeans <http://deerage.com/eagle_seed/> ------------------------------ Message: 3 Date: Mon, 15 Apr 2013 13:09:10 -0500 From: "DeMint, Valerie S" <valerie.dem...@phci.org> Subject: [Histonet] Thermohistoscreen Cassettes To: <histonet@lists.utsouthwestern.edu> Message-ID: <657b95ed46a98b4d9f44efc67edcaadd07477...@rwdex3.whs.phci.org> Content-Type: text/plain; charset="us-ascii" Is anyone waiting for their backorder of Thermo Histoscreen Cassettes? We no longer use this cassette and I am trying to sell my remaining inventory. I have several colors available. Write if you want more information :-) Valerie DeMint, HT(ASCP) Waukesha Memorial Hospital Waukesha WI This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 4 Date: Mon, 15 Apr 2013 14:14:28 -0400 From: Jack Ratliff <ratliffj...@hotmail.com> Subject: [Histonet] Bone, Biomaterials & Medical Device Implants Histology Event In Cambridge, MA - May 4th, 2013 To: Histonet <histonet@lists.utsouthwestern.edu> Cc: Jack Ratliff <jratl...@ratliffhistology.com>, Jack Ratliff <ratliffj...@gmail.com> Message-ID: <blu167-w18c069913a36ccbac3cd9fae...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" If you are currently working with bone, biomaterials or medical device implants, you won't want to miss this specialty histology event http://www.polysciences.com/Interactive-Histology-Forum-About/185/ sponsored by Polysciences Inc. and supported with six (6) continuing education units (CEU's) by the National Society for Histotechnology! There is still time to pre-register online http://events.r20.constantcontact.com/register/event?llr=gqebcrbab&oeidk=a07e6rs5o33629b055e or onsite May 4th at the Hyatt Regency Cambridge http://www.polysciences.com/Interactive-Histology-Forum-Hotel-Travel/189/. Topics of discussion will include: A Closer Look at Fixation, Decalcification and Processing Techniques for Paraffin Embedded SpecimensPresented by Robert A. Skinner, HTL (ASCP), Director of Laboratory Resources, Center for Orthopaedic Research, University of Arkansas for Medical Sciences Histology Technique Selection and Challenges for Biomaterial and Medical Device Implanted TissuesPresented by Philip Seifert, MS, HTL (ASCP), Principal Scientist, CBSET, Inc. Acrylic Resins: A Practical Approach for Demonstrating Undermineralized Bone, Biomaterials and Medical Device ImplantsPresented by Jack L. Ratliff, BA, Owner, Ratliff Histology Consultants & Chairman, Hard Tissue Committee National Society for Histotechnology The Use Of A Non-Contact Laser To Collect Thin Sections From A Variety of Soft And Hard Histological Tissues, Synthetic Bio-Resorbable Materials, and Tissues Containing Non-Resorbable Medical Device ImplantsPresented by Heiko Richter, Ph.D., Sales & Product Management, Rowiak GmbH Introduction to Routine Staining and Special Staining with Plastic Resin TechniquesPresented by Valantou Grover, HT (ASCP) HTL, PA, Product Line Manager, Business Manager, Histologist at Polysciences, Inc. If you have any questions, please feel free to contact myself or Ashley Gidzinski (ashley.gidzin...@polysciences.com). We look forward to seeing you in Cambridge, MA and May the 4th be with you! Best Regards, Jack Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology ------------------------------ Message: 5 Date: Mon, 15 Apr 2013 13:04:21 -0700 (PDT) From: Karla Arrington <freckles9...@yahoo.com> Subject: [Histonet] (no subject) To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1366056261.91439.yahoomail...@web121901.mail.ne1.yahoo.com> Content-Type: text/plain; charset=us-ascii How do I boost the power of mucicarmine for staining goblet cells? I have opened a new lot/bottle and have used full strength but it is not staining the cells. Thanks, Karla ------------------------------ Message: 6 Date: Mon, 15 Apr 2013 21:18:07 +0000 From: "Long, Denise" <denise.l...@uconn.edu> Subject: [Histonet] Glucose Oxidase blocking method To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <98ae5ddfdd7d204e9ce6c7ae28c0b27b0f72a...@maild.grove.ad.uconn.edu> Content-Type: text/plain; charset="us-ascii" Hello, I'm looking for updated information on the Glucose Oxidase blocking method. In particular, what reagents are being used now that Sigma's G5250 is no longer available. Does anyone know of a company that has this as a kit? Thanks, Denise Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ------------------------------ Message: 7 Date: Mon, 15 Apr 2013 15:12:23 -0700 From: Jennifer MacDonald <jmacdon...@mtsac.edu> Subject: [Histonet] Home based slide interpretation To: histonet@lists.utsouthwestern.edu Message-ID: <ofae011749.9e823dd0-on88257b4e.0079e2f2-88257b4e.0079e...@mtsac.edu> Content-Type: text/plain; charset="US-ASCII" Does anyone know of any regulations against a Pathologist reading slides from home? If so would you be able to cite the specific regulation? Thanks, Jennifer ------------------------------ Message: 8 Date: Mon, 15 Apr 2013 22:19:39 -0700 (PDT) From: khadija irfan <khadijair...@yahoo.com> Subject: [Histonet] Immunohistochemistry for Adipose tissue macrophages in humans To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <1366089579.51528.yahoomail...@web122204.mail.ne1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello All ? I am a clinical researcher so my questions may be somewhat naive, but I would be extremely grateful for any help. I want to look at adipose tissue macrophage polarity by immunohistochemistry for activation markers. I have the following questions: 1. How much tissue sample is required. I want to use subcutaneous and omental fat samples. 2. How should I transport and store my samples. How long can I store them? I anticipate that I would be collecting samples over 2-3 months. 3. If I do H&E staining first, can I do immunohistochemistry on the same slide? Can I do successive markers on the same slide? How many markers can I use it for? ? Thanks ? Khadija ------------------------------ Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald <jmacdon...@mtsac.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <1366119506.58355.yahoomail...@web163104.mail.bf1.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The only regulation about reading slides "from home" refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. From: Jennifer MacDonald <jmacdon...@mtsac.edu> To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 16 Apr 2013 16:30:14 +0200 (CEST) From: "Laura Avogaro" <avog...@science.unitn.it> Subject: [Histonet] Picrosirius red on cryosections To: histonet@lists.utsouthwestern.edu Message-ID: <2828.192.168.178.78.1366122614.squir...@www.science.unitn.it> Content-Type: text/plain;charset=iso-8859-1 Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) ? Italy Tel: +39 0461 283425 ------------------------------ Message: 11 Date: Tue, 16 Apr 2013 08:42:25 -0600 From: Elizabeth Chlipala <l...@premierlab.com> Subject: RE: [Histonet] Picrosirius red on cryosections To: Laura Avogaro <avog...@science.unitn.it>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <14E2C6176416974295479C64A11CB9AE016411AC2AE3@SBS2K8.premierlab.local> Content-Type: text/plain; charset="us-ascii" Laura This type of analysis does not need to be completed on frozen sections it can be performed on routine 10% NBF fixed paraffin sections. There are protocols on line on the website stains file. Here is the link http://stainsfile.info/StainsFile/stain/conektv/puchtler_psr.htm I would let the frozen sections air dry for at least 30 minutes and then post fix in 10% NBF for about 10 minutes and then start this procedure. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laura Avogaro Sent: Tuesday, April 16, 2013 8:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Picrosirius red on cryosections Hi everyone! I would like to stain cryosections of human atrial tissue with Picrosirius Red in order to quantify collagen fibers. Cryosections are obtained by a sample embedded in OCT compound without any fixative. Does anyone can suggest me a protocol to perform this staining? Thanks in advance Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Via delle Regole, 101 38123 Mattarello (TN) - Italy Tel: +39 0461 283425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 16 Apr 2013 11:32:41 -0400 From: "Pam Barker" <rel...@earthlink.net> Subject: [Histonet] RELIA Hot Job Alert 4-16-2013 Exciting Opportunities in NC.VA, GA, CA, CO, IL, TX and NM To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <0b2801ce3ab7$a2bec300$e83c4900$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, I hope you enjoyed a beautiful Spring weekend. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance and most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent The histology positions that I am most excited about are located in these areas: Charlotte, NC Salem, VA Staunton, VA Atlanta, GA Long Beach, CA Denver, CO Chicago, IL Springfield, IL Tyler, TX Waco, TX Roswell, NM Long Island, NY Syracuse, NY Louisville, KY East of Columbus, OH If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or rel...@earthlink.net or you can always catch me on cell via call or text at 407-353-5070. Thanks-Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia ------------------------------ Message: 13 Date: Tue, 16 Apr 2013 11:53:45 -0400 From: "Dessoye, Michael J" <mjdess...@commonwealthhealth.net> Subject: [Histonet] SP and CGRP antibodies To: "Histonet" <histonet@lists.utsouthwestern.edu> Message-ID: <e2547e1cd0ee324488a2940994571efa0549d...@wvhcs-exchange.wvhcs.com> Content-Type: text/plain; charset="us-ascii" Hello everyone, Is anyone out there using a commercially available antibody for SP (Substance P) and CGRP (Calcitonin gene-related peptide)? If so, where do you obtain it? Thanks! Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ------------------------------ Message: 14 Date: Tue, 16 Apr 2013 11:30:26 -0500 From: Deloris Carter <dels...@gmail.com> Subject: [Histonet] GSH Symposium To: histonet@lists.utsouthwestern.edu Message-ID: <caj6xiefkfwwyrov2hbb03aobea4cyy4bye+y38_z9kps5jx...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party! Deloris Carter ------------------------------ Message: 15 Date: Tue, 16 Apr 2013 12:52:11 -0400 From: "Shirley A. Powell" <powell...@mercer.edu> Subject: RE: [Histonet] GSH Symposium To: Deloris Carter <dels...@gmail.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <9BF995BC0E47744E9673A41486E24EE25799E77BB2@MERCERMAIL.MercerU.local> Content-Type: text/plain; charset="us-ascii" GSH would like to thank you for coming. Glad you had a good time and we will let you know when the next meeting is and the dates as soon as we get them. Crash our party anytime. Shirley Powell -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Tuesday, April 16, 2013 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GSH Symposium I'd like to say thank you to everyone at the GSH Symposium for making this "Yankee" feel welcome. I had a great time and found the sessions very helpful. Thanks again for letting me crash the party! Deloris Carter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 113, Issue 16 ***************************************** Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
